MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4101–4200 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4419 979.8223 2936.4451
4420 735.1296 2936.4893
4421 588.8974 2939.4506
4423 737.1299 2944.4905
4424 590.0861 2945.3941
4425 737.6025 2946.3809
4426 590.3339 2946.6331
4428 983.4814 2947.4224
4429 737.8820 2947.4989
4430 493.4120 2954.4283
4432 739.8636 2955.4253
4433 741.1255 2960.4729
4434 741.8792 2963.4877
4435 741.9335 2963.7049
4436 594.7673 2968.8001
4437 743.2118 2968.8181
4438 744.1150 2972.4309
4439 595.6944 2973.4356
4440 744.6170 2974.4389
4441 599.1399 2990.6631
4442 603.5718 3012.8226
4443 754.4661 3013.8353
4444 606.9493 3029.7101
4445 607.9443 3034.6851
4446 763.9456 3051.7533
4447 765.2247 3056.8697
4448 612.5775 3057.8511
4450 1025.5282 3073.5628
4451 770.6376 3078.5213
4452 616.7116 3078.5216
4453 616.7454 3078.6906
4454 616.7759 3078.8431
4455 1032.2397 3093.6973
4456 776.2308 3100.8941
4457 621.3839 3101.8831
4458 625.7787 3123.8571
4459 629.9878 3144.9026
4460 787.4890 3145.9269
4461 634.3604 3166.7656
4462 634.3845 3166.8861
4463 1061.8651 3182.5735
4464 796.6509 3182.5745
4465 532.3271 3187.9189
4466 638.7936 3188.9316
4467 798.2430 3188.9429
4468 643.1883 3210.9051
4469 539.8317 3232.9465
4470 647.8004 3233.9656
4472 651.9955 3254.9411
4473 656.4030 3276.9786
4474 547.3361 3277.9729
4475 663.9460 3314.6936
4476 554.5076 3321.0019
4477 665.2081 3321.0041
4478 1111.5510 3331.6312
4479 558.3411 3344.0029
4480 561.8448 3365.0251
4481 674.0140 3365.0336
4482 1124.6459 3370.9159
4483 309.0950 3388.9650
4484 682.8182 3409.0546
4485 569.3514 3410.0647
4486 1139.3213 3414.9421
4487 856.6869 3422.7185
4488 576.5207 3453.0805
4489 691.6243 3453.0851
4490 580.1854 3475.0687
4491 584.0253 3498.1081
4492 700.6304 3498.1156
4493 701.3964 3501.9456
4494 588.8612 3527.1235
4495 709.2334 3541.1306
4496 591.3621 3542.1289
4497 893.5093 3570.0081
4499 598.5333 3585.1561
4500 718.2393 3586.1601
4501 903.0272 3608.0797
4502 602.3648 3608.1451
4503 605.8714 3629.1847
4504 609.5392 3651.1915
4505 613.2086 3673.2079
4507 620.5464 3717.2347
4508 532.0409 3717.2354
4509 934.7023 3734.7801
4510 538.3304 3761.2619
4511 627.8880 3761.2843
4512 628.0521 3762.2689
4513 943.2481 3768.9633
4514 760.4077 3797.0021
4515 544.6205 3805.2926
4516 551.0526 3850.3173
4517 642.7275 3850.3213
4518 551.3384 3852.3179
4519 649.9030 3893.3743
4520 557.3439 3894.3564
4521 650.0692 3894.3715
4522 984.2603 3933.0121
4523 563.4897 3937.3770
4525 569.9221 3982.4038
4526 576.0682 4025.4265
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