MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4001–4100 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4300 644.8301 2575.2913
4301 859.4378 2575.2916
4302 859.7685 2576.2837
4303 859.7702 2576.2888
4304 517.2599 2581.2631
4305 861.4304 2581.2694
4306 646.5727 2582.2617
4307 863.4210 2587.2412
4308 863.4355 2587.2847
4309 864.0950 2589.2632
4310 648.5771 2590.2793
4311 864.7679 2591.2819
4312 864.7685 2591.2837
4313 648.8284 2591.2845
4314 519.8885 2594.4061
4315 649.6353 2594.5121
4316 866.7521 2597.2345
4317 520.4548 2597.2376
4318 650.3168 2597.2381
4319 650.3172 2597.2397
4321 866.7625 2597.2657
4322 867.0868 2598.2386
4324 868.7681 2603.2825
4325 869.1000 2604.2782
4326 869.4392 2605.2958
4327 653.8814 2611.4965
4328 523.6489 2613.2081
4330 872.0863 2613.2371
4331 523.6559 2613.2431
4332 872.0936 2613.2590
4333 654.5719 2614.2585
4334 655.1183 2616.4441
4335 874.0991 2619.2755
4336 874.4019 2620.1839
4337 874.4420 2620.3042
4338 656.3079 2621.2025
4339 877.4167 2629.2283
4343 879.1378 2634.3916
4344 659.8032 2635.1837
4345 528.0441 2635.1841
4346 879.4035 2635.1887
4347 880.0974 2637.2704
4348 880.1069 2637.2989
4349 528.6934 2638.4306
4350 660.6483 2638.5641
4351 662.3646 2645.4293
4352 664.8884 2655.5245
4353 666.1254 2660.4725
4354 666.1570 2660.5989
4355 888.1345 2661.3817
4357 889.4359 2665.2859
4358 889.4468 2665.3186
4359 893.4694 2677.3864
4360 537.4989 2682.4581
4362 896.7627 2687.2663
4366 675.8940 2699.5469
4367 677.1334 2704.5045
4368 677.1664 2704.6365
4370 682.3124 2725.2205
4371 909.7791 2726.3155
4372 546.3032 2726.4796
4373 682.6583 2726.6041
4377 688.1378 2748.5221
4378 688.1730 2748.6629
4379 692.8307 2767.2937
4382 697.9070 2787.5989
4383 699.1500 2792.5709
4384 699.1797 2792.6897
4385 559.5453 2792.6901
4387 562.9240 2809.5836
4388 563.9180 2814.5536
4389 707.3755 2825.4729
4390 708.9135 2831.6249
4391 710.1567 2836.5977
4392 568.3502 2836.7146
4393 710.1861 2836.7153
4394 949.4835 2845.4287
4395 571.7285 2853.6061
4396 572.7186 2858.5566
4397 956.1227 2865.3463
4398 719.9196 2875.6493
4399 721.1926 2880.7413
4400 577.1559 2880.7431
4401 581.5236 2902.5816
4402 970.1544 2907.4414
4403 970.1562 2907.4468
4404 730.1271 2916.4793
4405 973.1686 2916.4840
4406 730.1286 2916.4853
4407 730.1296 2916.4893
4408 730.9263 2919.6761
4409 732.1991 2924.7673
4410 585.9617 2924.7721
4411 733.3620 2929.4189
4412 587.0905 2930.4161
4413 587.2912 2931.4196
4414 734.1267 2932.4777
4416 734.8771 2935.4793
4417 734.8788 2935.4861
4418 979.5044 2935.4914
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