MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3901–4000 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4164 780.0833 2337.2281
4165 780.4141 2338.2205
4167 587.8431 2347.3433
4168 589.0807 2352.2937
4169 589.1107 2352.4137
4170 785.4136 2353.2190
4171 787.4230 2359.2472
4172 790.4130 2368.2172
4175 791.4042 2371.1908
4176 792.4517 2374.3333
4177 594.5915 2374.3369
4178 594.6095 2374.4089
4179 1194.1389 2386.2632
4188 597.6113 2386.4161
4189 598.8497 2391.3697
4190 600.0696 2396.2493
4191 600.0870 2396.3189
4192 600.1202 2396.4517
4193 800.4161 2398.2265
4195 803.7441 2408.2105
4197 482.8553 2409.2401
4198 603.3177 2409.2417
4200 604.5723 2414.2601
4201 805.7609 2414.2609
4202 604.5725 2414.2609
4203 805.7626 2414.2660
4204 806.7567 2417.2483
4205 484.4662 2417.2946
4206 807.1270 2418.3592
4207 405.0428 2424.2131
4208 607.0609 2424.2145
4209 485.8502 2424.2146
4210 809.0798 2424.2176
4212 485.8511 2424.2191
4213 486.8522 2429.2246
4214 811.0830 2430.2272
4215 608.5645 2430.2289
4217 488.2543 2436.2351
4218 813.1229 2436.3469
4219 813.1302 2436.3688
4220 610.1072 2436.3997
4223 611.0932 2440.3437
4224 611.1234 2440.4645
4226 611.5747 2442.2697
4227 611.8311 2443.2953
4228 612.0790 2444.2869
4229 614.0583 2452.2041
4230 491.4482 2452.2046
4231 616.2950 2461.1509
4232 493.4393 2462.1601
4233 493.4727 2462.3271
4234 821.8017 2462.3833
4235 616.6098 2462.4101
4236 616.6183 2462.4441
4237 494.6424 2468.1756
4238 620.8632 2479.4237
4241 622.0992 2484.3677
4242 622.1299 2484.4905
4243 829.7682 2486.2828
4244 830.0964 2487.2674
4245 830.7501 2489.2285
4246 830.7516 2489.2330
4247 832.0812 2493.2218
4248 832.0820 2493.2242
4249 832.0822 2493.2248
4252 836.0798 2505.2176
4253 502.2779 2506.3531
4254 627.6117 2506.4177
4255 627.6236 2506.4653
4258 839.0984 2514.2734
4259 629.8066 2515.1973
4260 839.4081 2515.2025
4262 840.0831 2517.2275
4264 631.0776 2520.2813
4265 505.2602 2521.2646
4266 505.2632 2521.2796
4267 631.5732 2522.2637
4268 841.7779 2522.3119
4269 631.8691 2523.4473
4270 632.0649 2524.2305
4271 842.4178 2524.2316
4272 505.8537 2524.2321
4273 632.8024 2527.1805
4274 506.4434 2527.1806
4275 633.1063 2528.3961
4276 844.1170 2529.3292
4277 633.8002 2531.1717
4278 633.8004 2531.1725
4279 844.7327 2531.1763
4281 508.4596 2537.2616
4282 511.0828 2550.3776
4283 638.6314 2550.4965
4284 511.6576 2553.2516
4289 854.1037 2559.2893
4293 855.4374 2563.2904
4294 856.4307 2566.2703
4296 642.8759 2567.4745
4297 644.1129 2572.4225
4298 644.1436 2572.5453
4299 859.4377 2575.2913
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