MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2527 502.7593 1003.5040 1003.5087 -4.60 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology		 VAAAQYEPR
3120 460.5625 1378.6657 1378.6881 -16.3 0 1 3.5 +1Score > 19 indicates identity FPYVNANVIDAR + Deamidated (NQ)
4320 650.3234 2597.2645 2597.2292 13.6 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology		 TQQLRQMNQHNGWLLEGQIER + 3 Deamidated (NQ); Oxidation (M)
4427 983.4780 2947.4122 2947.3546 19.5 0 1 0.81 +1Score > 20 indicates identity
Score > 13 indicates homology		 VVPDAGNLLAQQAIADVFCVNGDSEWR + 4 Deamidated (NQ)
2197 360.7057 719.3968 719.3966 0.31 0 1 2.4 +1Score > 17 indicates identity GEVLFR
2543 506.7380 1011.4614 1011.4734 -11.8 1 1 1.4 +1Score > 15 indicates identity DEPGPGRER
4089 419.2068 2090.9976 2090.9976 0.015 1 1 3.5 +1Score > 19 indicates identity AMNLSEDGVLVMQLEQRR + 3 Deamidated (NQ)
2967 654.3096 1306.6046 1306.6261 -16.4 0 1 2.1 +1Score > 17 indicates identity ELALLMSDAGCK
3771 468.0064 1867.9965 1868.0189 -12.0 0 1 2.2 +1Score > 17 indicates identity VRPGILAVEALNLMQSR + 2 Deamidated (NQ)
4257 628.3188 2509.2461 2509.2925 -18.5 1 1 3.6 +1Score > 19 indicates identity SQGSEFDHAALILPSQRTPVVTR + Deamidated (NQ)
3599 586.2822 1755.8248 1755.8540 -16.6 1 1 2.3 +1Score > 17 indicates identity AQGKYPVHLSGGQQQR + 3 Deamidated (NQ)
4285 639.5712 2554.2557 2554.2560 -0.11 1 1 3.6 +1Score > 19 indicates identity QISTIYQPMNQYKVVMEVDPR + Oxidation (M)
4056 687.6719 2059.9939 2059.9527 20.0 0 1 4 +1Score > 20 indicates identity GYYGWDFINDTQILTPR + 2 Deamidated (NQ)
2351 901.4757 900.4684 900.4665 2.18 1 1 2.7 +1Score > 18 indicates identity KEIPSGNR + Deamidated (NQ)
4047 410.4022 2046.9746 2046.9761 -0.73 1 1 3.4 +1Score > 19 indicates identity ENIMNNPVIGVVMCRNR + 2 Oxidation (M)
3588 877.4192 1752.8238 1752.8206 1.84 0 1 3.1 +1Score > 18 indicates identity LYDADLPEYNVAVDR + Deamidated (NQ)
4381 555.1091 2770.5091 2770.4640 16.3 1 1 1.1 +1Score > 14 indicates identity PIIQSVERALQILDLFNEQATELK + 3 Deamidated (NQ)
2536 1010.4874 1009.4801 1009.4651 14.9 0 1 1.7 +1Score > 16 indicates identity GMVFEQQR + Oxidation (M)
2557 510.7740 1019.5334 1019.5287 4.61 0 1 3.4 +1Score > 19 indicates identity LFEEQVVR + Deamidated (NQ)
2567 515.3030 1028.5914 1028.5978 -6.21 0 1 2.6 +1Score > 18 indicates identity LVLTTQAQR
4061 516.7440 2062.9469 2062.9629 -7.76 1 1 3.5 +1Score > 19 indicates identity SNIINLEWQQIDMQRR + 4 Deamidated (NQ); Oxidation (M)
4044 512.5015 2045.9769 2045.9389 18.6 0 1 3.3 +1Score > 19 indicates identity LEAAVAQSEQQGEAALSDAR + 3 Deamidated (NQ)
2751 596.3229 1190.6312 1190.6520 -17.4 1 1 3.7 +1Score > 19 indicates identity DLLRDPGLHR
3752 617.6633 1849.9681 1849.9549 7.12 0 1 3.2 +1Score > 18 indicates identity AFTNYLPGFNIPMLPR
2224 743.4437 742.4364 742.4225 18.8 0 1 3.8 +1Score > 19 indicates identity DATPIVK
3621 442.7279 1766.8825 1766.8621 11.5 1 1 0.86 +1Score > 20 indicates identity
Score > 13 indicates homology		 MVQDAFSKVVSQADAR + Oxidation (M)
2858 629.2505 1256.4864 1256.4728 10.9 0 1 0.82 +1Score > 13 indicates identity GDYTCGGQDQR + Deamidated (NQ)
4364 675.8395 2699.3289 2699.3397 -4.01 0 1 3.4 +1Score > 19 indicates identity EDQPMMTQLLLLPLLQQLGQQSR + 4 Deamidated (NQ); Oxidation (M)
3776 624.0288 1869.0646 1869.0683 -2.00 1 1 0.83 +1Score > 13 indicates identity RIQILENTLATTLLNR + Deamidated (NQ)
2231 374.7048 747.3950 747.3915 4.69 0 1 2.6 +1Score > 17 indicates identity LTPFDR
3246 478.2309 1431.6709 1431.6956 -17.2 0 1 2.8 +1Score > 18 indicates identity VPETMPPQLFEK + Deamidated (NQ); Oxidation (M)
4341 526.8538 2629.2326 2629.2368 -1.60 1 1 3.6 +1Score > 19 indicates identity TTEQSWQQQTRTSQAAPVQAQPR + 3 Deamidated (NQ)
2863 419.8536 1256.5390 1256.5408 -1.47 0 1 1.1 +1Score > 14 indicates identity TTITEYNNDGK + 2 Deamidated (NQ)
3837 960.4236 1918.8326 1918.8400 -3.85 0 1 1.4 +1Score > 15 indicates identity NCQQAIAQGIAMVPEDR + 3 Deamidated (NQ); Oxidation (M)
2727 583.8338 1165.6530 1165.6455 6.48 0 1 1 +1Score > 13 indicates identity AQLSLQHIQK + Deamidated (NQ)
2555 510.7728 1019.5310 1019.5321 -1.05 0 1 3.8 +1Score > 19 indicates identity DMVLATLNK + Oxidation (M)
3409 814.4247 1626.8348 1626.8366 -1.05 1 1 3.3 +1Score > 18 indicates identity LDQHGGFIRQVLDK + 2 Deamidated (NQ)
3502 572.3325 1713.9757 1713.9600 9.17 1 1 1 +1Score > 13 indicates identity ILLIMGEWLAVQRR + Deamidated (NQ); Oxidation (M)
4261 629.8366 2515.3173 2515.3138 1.38 1 1 2.8 +1Score > 18 indicates identity RMISGEMDVVLVPQGTLIEQIR + 2 Oxidation (M)
3091 342.1942 1364.7477 1364.7663 -13.7 0 1 1.3 +1Score > 14 indicates identity EIAHLTDKPTLK
3853 644.0499 1929.1279 1929.1299 -1.03 0 1 0.87 +1Score > 13 indicates identity FLSALILLLVTTAAQAER
4140 563.2704 2249.0525 2249.0851 -14.5 1 1 3.5 +1Score > 19 indicates identity QSNLVEKLESLQPGYNYHK + 3 Deamidated (NQ)
3985 672.3401 2013.9985 2014.0346 -17.9 0 1 3.8 +1Score > 19 indicates identity IATPLGPLWVICDEQFR
2825 311.1370 1240.5189 1240.5142 3.76 1 1 0.92 +1Score > 13 indicates identity EFDRQMDER + Oxidation (M)
3759 465.7369 1858.9185 1858.9346 -8.66 1 1 4.4 +1Score > 20 indicates identity AAGAELVGMEDLADQIKK + Deamidated (NQ)
2288 415.2317 828.4488 828.4593 -12.6 0 1 2.6 +1Score > 17 indicates identity ITPLEEK
2546 507.7619 1013.5092 1013.5142 -4.85 0 1 2.5 +1Score > 17 indicates identity LPGELSGGQR + Deamidated (NQ)
2438 318.5052 952.4938 952.4800 14.4 1 1 2.5 +1Score > 17 indicates identity FGGMKIER + Oxidation (M)
3713 610.6468 1828.9186 1828.9108 4.27 1 1 4.1 +1Score > 19 indicates identity WLDNEGKFRPENPVK + Deamidated (NQ)
2676 558.7719 1115.5292 1115.5458 -14.9 0 1 2.8 +1Score > 17 indicates identity QIDDQLLNR + 2 Deamidated (NQ)
2794 408.1989 1221.5749 1221.5989 -19.7 1 1 3.5 +1Score > 18 indicates identity QRSVFAEGAEK + Deamidated (NQ)
3497 572.3309 1713.9709 1713.9600 6.37 1 1 1.1 +1Score > 13 indicates identity ILLIMGEWLAVQRR + Deamidated (NQ); Oxidation (M)
3138 463.5762 1387.7068 1387.6819 17.9 0 0 4.1 +1Score > 19 indicates identity NWYLLICNHR
2736 394.2086 1179.6040 1179.6261 -18.8 1 0 2.9 +1Score > 18 indicates identity DHRNWLALR
3841 640.9741 1919.9005 1919.8968 1.90 0 0 3.6 +1Score > 19 indicates identity DVRPPAELISSMNAQMK + 2 Deamidated (NQ); 2 Oxidation (M)
3141 694.8666 1387.7186 1387.7347 -11.6 0 0 4.2 +1Score > 19 indicates identity SALNNFLEPLLR + 2 Deamidated (NQ)
3691 607.9953 1820.9641 1821.0004 -20.0 1 0 3 +1Score > 18 indicates identity HMELLAHSILKLICK + Oxidation (M)
2749 595.3221 1188.6296 1188.6437 -11.8 1 0 3.8 +1Score > 19 indicates identity RWNMLLSLR + Deamidated (NQ)
4365 900.7844 2699.3314 2699.3312 0.067 1 0 3.8 +1Score > 19 indicates identity AMELCRPLPWIDDDKPRLGDFR
3018 668.8227 1335.6308 1335.6531 -16.7 1 0 3.6 +1Score > 18 indicates identity QHNTLSKHNQK + 2 Deamidated (NQ)
2677 372.8510 1115.5312 1115.5459 -13.2 0 0 2.8 +1Score > 17 indicates identity QLDDLEVQR + Deamidated (NQ)
2977 657.3591 1312.7036 1312.6987 3.78 0 0 2.9 +1Score > 18 indicates identity QGATPLVVVEGSR + Deamidated (NQ)
3605 440.2393 1756.9281 1756.9505 -12.8 1 0 3.6 +1Score > 18 indicates identity STDNLPLREAIMLLR + Oxidation (M)
2437 318.4972 952.4698 952.4726 -2.98 0 0 2.3 +1Score > 16 indicates identity NLVENAHR + Deamidated (NQ)
4063 689.6917 2066.0533 2066.0717 -8.93 1 0 3.4 +1Score > 18 indicates identity RDAMQSLLLPPPAQQALAK + 3 Deamidated (NQ); Oxidation (M)
3696 608.3314 1821.9724 1821.9658 3.59 1 0 2.7 +1Score > 17 indicates identity MSGTVKLGYQRPLNIK + 2 Deamidated (NQ); Oxidation (M)
2821 310.8900 1239.5309 1239.5367 -4.72 0 0 0.97 +1Score > 13 indicates identity FNSSLSEDGQR + Deamidated (NQ)
2190 359.2047 716.3948 716.3817 18.4 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology		 GLTQAAR + Deamidated (NQ)
2744 395.8796 1184.6170 1184.6149 1.74 1 0 3.3 +1Score > 18 indicates identity DLPASERELR
3504 572.3337 1713.9793 1713.9600 11.3 1 0 0.97 +1Score > 13 indicates identity ILLIMGEWLAVQRR + Deamidated (NQ); Oxidation (M)
3981 1005.5224 2009.0302 2008.9928 18.6 1 0 4.1 +1Score > 19 indicates identity TISQLYDPEKGMFLPDR
4072 692.6846 2075.0320 2075.0660 -16.4 1 0 0.94 +1Score > 20 indicates identity
Score > 13 indicates homology		 LLEQAQEWPQPDGRPRR
2820 619.2965 1236.5784 1236.5986 -16.3 1 0 3.3 +1Score > 18 indicates identity KTNVFNEQVR + 3 Deamidated (NQ)
3009 443.8768 1328.6086 1328.6105 -1.43 0 0 1.7 +1Score > 15 indicates identity LFVMMTLGDDR + 2 Oxidation (M)
3945 501.0288 2000.0861 2000.0612 12.4 1 0 1.8 +1Score > 15 indicates identity KIVLALGGNALGDDLAGQMK + Deamidated (NQ); Oxidation (M)
2426 315.4810 943.4212 943.4399 -19.8 1 0 1.1 +1Score > 13 indicates identity YEYAKNR + Deamidated (NQ)
2732 391.8580 1172.5522 1172.5747 -19.2 0 0 2.1 +1Score > 16 indicates identity NIAGMVDLNPK + 2 Deamidated (NQ)
4042 1023.9916 2045.9686 2046.0051 -17.8 0 0 3.7 +1Score > 19 indicates identity ENVAALNINLSDADATLMR + Oxidation (M)
3171 349.9416 1395.7373 1395.7146 16.2 1 0 2.7 +1Score > 17 indicates identity RYYSLTTHNLK + Deamidated (NQ)
4329 654.3094 2613.2085 2613.2315 -8.82 1 0 4 +1Score > 19 indicates identity AISWSTDYQGMVNGILSGNGKQMR + Deamidated (NQ)
2364 304.8355 911.4847 911.5018 -18.7 1 0 1.6 +1Score > 15 indicates identity FFFAPKR
3865 484.9809 1935.8945 1935.8646 15.5 1 0 3.4 +1Score > 18 indicates identity QNSRTCYLSWHEAAGR + Deamidated (NQ)
2877 633.3675 1264.7204 1264.7431 -17.9 0 0 0.99 +1Score > 13 indicates identity TFFTVIVPLTK
3929 663.3433 1987.0081 1986.9799 14.2 0 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology		 HYPLEADNIFAAIAATNR + Deamidated (NQ)
4174 791.0797 2370.2173 2370.1864 13.0 0 0 3.5 +1Score > 18 indicates identity DLNQELMRPHVFPFPLLMR + 2 Deamidated (NQ); Oxidation (M)
3570 874.9022 1747.7898 1747.8199 -17.2 0 0 2.9 +1Score > 17 indicates identity IDVDEFSHCLSASLR
2743 395.8716 1184.5930 1184.5720 17.7 0 0 3 +1Score > 17 indicates identity MQHQVNVSAR + Oxidation (M)
2965 653.3654 1304.7162 1304.6949 16.3 1 0 2.8 +1Score > 17 indicates identity TNPVTGRVAPHR + Deamidated (NQ)
4018 1019.5179 2037.0212 2037.0418 -10.1 1 0 4.6 +1Score > 19 indicates identity WVKQISEELHLDEILNA + Deamidated (NQ)
2483 328.4836 982.4290 982.4212 7.94 0 0 1.5 +1Score > 14 indicates identity VMSMNASAR + Deamidated (NQ); Oxidation (M)
3270 484.9032 1451.6878 1451.7047 -11.7 1 0 3.3 +1Score > 18 indicates identity MLTRGMCVELAR + Oxidation (M)
2910 640.3130 1278.6114 1278.5948 13.0 0 0 4.2 +1Score > 19 indicates identity MMQLTGNPEVK + 2 Oxidation (M)
2435 476.2516 950.4886 950.4756 13.7 1 0 3.8 +1Score > 18 indicates identity KMDAHPPR
3977 503.2475 2008.9609 2008.9683 -3.70 0 0 4.9 +1Score > 20 indicates identity WDFLHFGLTGTSAFDPAK
4071 416.0015 2074.9711 2074.9849 -6.65 1 0 3.7 +1Score > 18 indicates identity MIKENQLMMLAHGVASPR + 2 Deamidated (NQ); 3 Oxidation (M)
4356 888.4694 2662.3864 2662.3901 -1.39 1 0 3.3 +1Score > 18 indicates identity AHNSGHEVLIHLPMAPLSKQPLEK + Deamidated (NQ); Oxidation (M)
3408 543.2846 1626.8320 1626.8512 -11.8 1 0 4.1 +1Score > 19 indicates identity MLQQIVTTAHQRGK + Deamidated (NQ); Oxidation (M)
2854 628.3631 1254.7116 1254.7044 5.78 1 0 1.9 +1Score > 15 indicates identity ERQAIEQLIR
3352 769.4070 1536.7994 1536.7752 15.8 1 0 2.8 +1Score > 17 indicates identity MISNAVNRLMISR + Deamidated (NQ); 2 Oxidation (M)
2489 492.7168 983.4190 983.4165 2.63 0 0 1.1 +1Score > 13 indicates identity TTCSLCSR
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