MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4036 511.8198 2043.2501
4037 512.0345 2044.1089
4038 682.4053 2044.1941
4039 682.4163 2044.2271
4040 682.4241 2044.2505
4041 512.2666 2045.0373
4043 512.5005 2045.9729
4045 683.0203 2046.0391
4046 683.3318 2046.9736
4048 683.3336 2046.9790
4049 685.3583 2053.0531
4052 687.0410 2058.1012
4053 687.3466 2059.0180
4054 1030.5210 2059.0274
4055 515.7905 2059.1329
4057 516.0248 2060.0701
4058 516.2704 2061.0525
4059 688.0751 2061.2035
4062 517.3104 2065.2125
4064 517.5455 2066.1529
4065 689.7250 2066.1532
4067 690.3254 2067.9544
4073 520.2612 2077.0157
4074 520.4995 2077.9689
4075 521.2864 2081.1165
4077 521.8062 2083.1957
4078 695.9800 2084.9182
4079 522.2383 2084.9241
4080 697.0258 2088.0556
4081 523.0414 2088.1365
4082 697.0723 2088.1951
4083 1045.1068 2088.1990
4084 523.0750 2088.2709
4085 697.0977 2088.2713
4086 697.1006 2088.2800
4087 697.9869 2090.9389
4088 523.7425 2090.9409
4090 523.7568 2090.9981
4091 698.3395 2091.9967
4092 525.2513 2096.9761
4093 526.8001 2103.1713
4094 702.7508 2105.2306
4095 704.0662 2109.1768
4096 528.3173 2109.2401
4097 528.5522 2110.1797
4098 528.5713 2110.2561
4099 529.7888 2115.1261
4100 532.2927 2125.1417
4101 532.8129 2127.2225
4102 710.3334 2127.9784
4104 533.8281 2131.2833
4105 711.4401 2131.2985
4107 711.7727 2132.2963
4108 715.7081 2144.1025
4109 537.8027 2147.1817
4110 717.4256 2149.2550
4111 718.7410 2153.2012
4112 539.5593 2154.2081
4113 539.5755 2154.2729
4114 543.2991 2169.1673
4115 1086.5781 2171.1416
4116 724.7212 2171.1418
4117 543.8149 2171.2305
4118 725.0399 2172.0979
4119 545.0546 2176.1893
4120 726.4440 2176.3102
4121 545.0864 2176.3165
4122 546.5201 2182.0513
4123 732.1006 2193.2800
4124 733.4160 2197.2262
4125 550.3356 2197.3133
4126 550.5642 2198.2277
4127 735.0554 2202.1444
4130 737.3699 2209.0879
4132 554.8251 2215.2713
4133 556.0613 2220.2161
4134 556.0924 2220.3405
4135 741.1236 2220.3490
4136 559.8198 2235.2501
4137 746.7756 2237.3050
4138 748.4255 2242.2547
4139 561.5734 2242.2645
4141 752.0740 2253.2002
4142 565.3120 2257.2189
4143 565.5774 2258.2805
4144 565.8314 2259.2965
4146 567.0980 2264.3629
4147 568.2706 2269.0533
4149 761.4510 2281.3312
4150 572.3271 2285.2793
4151 763.1018 2286.2836
4152 572.5942 2286.3477
4156 576.8372 2303.3197
4157 578.0743 2308.2681
4158 578.1044 2308.3885
4159 578.7897 2311.1297
4160 776.1262 2325.3568
4161 777.4418 2329.3036
4162 583.5817 2330.2977
4163 583.6060 2330.3949
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