MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3907 493.7613 1971.0161
3908 493.7797 1971.0897
3909 658.3870 1972.1392
3910 659.6819 1976.0239
3911 659.9415 1976.8027
3912 495.2080 1976.8029
3913 396.3781 1976.8541
3914 495.2811 1977.0953
3915 660.0393 1977.0961
3916 495.2975 1977.1609
3917 495.4717 1977.8577
3918 660.2937 1977.8593
3919 660.3672 1978.0798
3920 660.3968 1978.1686
3921 496.2520 1980.9789
3924 662.0297 1983.0673
3925 994.0078 1986.0010
3926 663.3362 1986.9868
3928 663.3405 1986.9997
3930 994.5124 1987.0102
3931 663.3444 1987.0114
3932 499.2733 1993.0641
3933 665.3856 1993.1350
3934 665.6938 1994.0596
3935 499.7891 1995.1273
3936 499.9891 1995.9273
3937 499.9966 1995.9573
3938 500.0110 1996.0149
3939 666.3463 1996.0171
3940 666.6593 1996.9561
3941 400.3987 1996.9571
3942 500.2466 1996.9573
3943 667.3438 1999.0096
3944 500.7769 1999.0785
3946 667.7147 2000.1223
3947 1001.0795 2000.1444
3948 667.7285 2000.1637
3950 667.7455 2000.2147
3951 501.0627 2000.2217
3955 501.4991 2001.9673
3956 668.3657 2002.0753
3957 501.5261 2002.0753
3958 1002.0463 2002.0780
3959 1002.0464 2002.0782
3960 668.3667 2002.0783
3961 668.3675 2002.0807
3962 668.3677 2002.0813
3963 668.3678 2002.0816
3964 1002.0482 2002.0818
3965 668.3683 2002.0831
3966 668.3685 2002.0837
3967 668.3686 2002.0840
3968 668.3686 2002.0840
3969 668.3688 2002.0846
3970 668.3691 2002.0855
3971 668.3724 2002.0954
3972 668.6648 2002.9726
3973 669.3693 2005.0861
3978 503.2619 2009.0185
3982 670.6846 2009.0320
3983 670.6853 2009.0341
3984 670.6860 2009.0362
3987 504.7840 2015.1069
3988 673.0186 2016.0340
3990 673.4006 2017.1800
3992 505.7422 2018.9397
3993 674.7147 2021.1223
3994 675.0221 2022.0445
3995 675.0424 2022.1054
3996 506.5387 2022.1257
3997 506.5559 2022.1945
4000 675.3593 2023.0561
4001 507.0191 2024.0473
4002 507.0200 2024.0509
4003 675.6931 2024.0575
4004 507.2416 2024.9373
4005 507.2455 2024.9529
4006 676.3218 2025.9436
4007 677.3650 2029.0732
4009 1016.0413 2030.0680
4010 677.6978 2030.0716
4011 677.7001 2030.0785
4013 678.3362 2031.9868
4017 679.6821 2036.0245
4020 510.2795 2037.0889
4021 680.6817 2039.0233
4022 510.7646 2039.0293
4023 510.7991 2039.1673
4024 1021.0187 2040.0228
4025 681.0156 2040.0250
4026 409.0124 2040.0256
4027 409.0124 2040.0256
4028 511.0140 2040.0269
4029 511.0141 2040.0273
4030 511.0147 2040.0297
4031 681.6528 2041.9366
4032 681.9973 2042.9701
4033 682.0571 2043.1495
4034 1022.5896 2043.1646
4035 682.0872 2043.2398
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