MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3781 625.3450 1873.0132
3784 938.0764 1874.1382
3786 938.5641 1875.1136
3787 938.9384 1875.8622
3788 626.9634 1877.8684
3789 470.7283 1878.8841
3792 628.6834 1883.0284
3793 471.7647 1883.0297
3794 629.3733 1885.0981
3795 629.6298 1885.8676
3796 630.6862 1889.0368
3797 473.2673 1889.0401
3798 630.6895 1889.0467
3799 630.7133 1889.1181
3800 630.9631 1889.8675
3801 946.4421 1890.8696
3803 946.4432 1890.8718
3804 631.2985 1890.8737
3805 631.2985 1890.8737
3806 474.4595 1893.8089
3807 475.2177 1896.8417
3808 949.4351 1896.8556
3810 634.9634 1901.8684
3811 952.4431 1902.8716
3812 952.4441 1902.8736
3813 477.2578 1905.0021
3814 636.0305 1905.0697
3818 954.4390 1906.8634
3819 954.4398 1906.8650
3820 477.7797 1907.0897
3821 956.5504 1911.0862
3822 638.0601 1911.1585
3823 478.7971 1911.1593
3824 638.0607 1911.1603
3825 478.9624 1911.8205
3826 638.2942 1911.8608
3827 479.0121 1912.0193
3828 638.3683 1912.0831
3829 638.3747 1912.1023
3830 957.4165 1912.8184
3831 638.6140 1912.8202
3832 638.6147 1912.8223
3833 479.2662 1913.0357
3834 638.7045 1913.0917
3836 640.2842 1917.8308
3838 960.4379 1918.8612
3839 960.4403 1918.8660
3840 960.9433 1919.8720
3842 480.9827 1919.9017
3843 962.4354 1922.8562
3845 641.9603 1922.8591
3846 643.3591 1927.0555
3847 482.7729 1927.0625
3848 643.6038 1927.7896
3849 643.6130 1927.8172
3850 482.9619 1927.8185
3851 482.9620 1927.8189
3852 643.9472 1928.8198
3854 644.2823 1929.8251
3855 645.3560 1933.0462
3856 645.3632 1933.0678
3857 645.3633 1933.0681
3858 484.2749 1933.0705
3859 484.2925 1933.1409
3860 645.7217 1934.1433
3861 645.9360 1934.7862
3862 968.4377 1934.8608
3863 968.4388 1934.8630
3866 485.2023 1936.7801
3867 969.9333 1937.8520
3869 970.4316 1938.8486
3870 647.3100 1938.9082
3871 485.7348 1938.9101
3873 486.9601 1943.8113
3874 649.2775 1944.8107
3878 488.2662 1949.0357
3879 650.7089 1949.1049
3880 650.9290 1949.7652
3881 488.4486 1949.7653
3882 488.5327 1950.1017
3883 976.4337 1950.8528
3884 976.9404 1951.8662
3885 489.7673 1955.0401
3886 489.7701 1955.0513
3887 978.5629 1955.1112
3888 652.7345 1955.1817
3889 652.7346 1955.1820
3890 652.9345 1955.7817
3891 653.0438 1956.1096
3892 653.0518 1956.1336
3893 490.0550 1956.1909
3895 653.4029 1957.1869
3899 654.0113 1959.0121
3900 654.0117 1959.0133
3901 490.9575 1959.8009
3902 654.2755 1959.8047
3903 655.0300 1962.0682
3904 491.7248 1962.8701
3905 655.2982 1962.8728
3906 658.0111 1971.0115
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