MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3538 579.6701 1735.9885
3539 579.6783 1736.0131
3544 580.2609 1737.7609
3545 869.9393 1737.8640
3546 580.5898 1738.7476
3547 870.3851 1738.7556
3548 870.3922 1738.7698
3549 871.4155 1740.8164
3550 581.2802 1740.8188
3551 581.3170 1740.9292
3552 871.4879 1740.9612
3553 871.4903 1740.9660
3554 871.4920 1740.9694
3555 871.9116 1741.8086
3556 581.6104 1741.8094
3557 581.6631 1741.9675
3558 581.6639 1741.9699
3559 581.6643 1741.9711
3560 581.6646 1741.9720
3561 581.6666 1741.9780
3562 872.4139 1742.8132
3563 872.9011 1743.7876
3564 873.3905 1744.7664
3565 873.4874 1744.9602
3566 873.4876 1744.9606
3567 873.4887 1744.9628
3568 873.8903 1745.7660
3569 583.3612 1747.0618
3571 583.9387 1748.7943
3572 584.2680 1749.7822
3577 876.4645 1750.9144
3578 584.6464 1750.9174
3582 584.6562 1750.9468
3583 876.9650 1751.9154
3584 351.3907 1751.9171
3585 438.9880 1751.9229
3586 438.9881 1751.9233
3587 585.0098 1752.0076
3589 877.8733 1753.7320
3590 585.5862 1753.7368
3591 585.9143 1754.7211
3592 878.3680 1754.7214
3593 585.9172 1754.7298
3594 878.3735 1754.7324
3595 878.4148 1754.8150
3596 585.9520 1754.8342
3597 878.4244 1754.8342
3600 878.9203 1755.8260
3602 879.4191 1756.8236
3603 586.6485 1756.9237
3604 440.2383 1756.9241
3606 879.4717 1756.9288
3607 586.6611 1756.9615
3608 586.6837 1757.0293
3609 586.9961 1757.9665
3612 588.3278 1761.9616
3613 441.7006 1762.7733
3614 882.8778 1763.7410
3615 882.8862 1763.7578
3616 588.9277 1763.7613
3617 441.9479 1763.7625
3618 588.9335 1763.7787
3619 588.9961 1763.9665
3620 589.2615 1764.7627
3622 589.9693 1766.8861
3624 590.9060 1769.6962
3625 591.0045 1769.9917
3626 591.0050 1769.9932
3627 591.3370 1770.9892
3628 887.4521 1772.8896
3629 591.9834 1772.9284
3630 592.0045 1772.9917
3631 444.4897 1773.9297
3633 592.6496 1774.9270
3634 592.6967 1775.0683
3635 445.7440 1778.9469
3636 594.0316 1779.0730
3637 594.2579 1779.7519
3638 594.3125 1779.9157
3640 594.3410 1780.0012
3641 891.0108 1780.0070
3642 594.3475 1780.0207
3643 594.3535 1780.0387
3644 891.0454 1780.0762
3645 594.3666 1780.0780
3646 594.6782 1781.0128
3647 892.9163 1783.8180
3648 595.9938 1784.9596
3649 596.9962 1787.9668
3650 597.2928 1788.8566
3651 448.2215 1788.8569
3654 895.9970 1789.9794
3655 598.0374 1791.0904
3656 598.2354 1791.6844
3657 449.1793 1792.6881
3658 449.4504 1793.7725
3659 598.9315 1793.7727
3660 449.4633 1793.8241
3661 598.9487 1793.8243
3662 599.2982 1794.8728
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