MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2701–2800 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2500 987.5975 986.5902
2501 494.3033 986.5920
2502 494.3034 986.5922
2516 497.2360 992.4574
2517 497.2774 992.5402
2519 333.8314 998.4724
2520 500.2447 998.4748
2521 501.2495 1000.4844
2526 502.2656 1002.5166
2528 335.8091 1004.4055
2529 504.2810 1006.5474
2530 336.8281 1007.4625
2531 337.1493 1008.4261
2532 337.1579 1008.4519
2533 505.2951 1008.5756
2537 505.8116 1009.6086
2542 338.1605 1011.4597
2547 339.1778 1014.5116
2548 508.2693 1014.5240
2549 508.7076 1015.4006
2551 508.7567 1015.4988
2552 508.7570 1015.4994
2553 339.8280 1016.4622
2554 509.2393 1016.4640
2556 1020.5394 1019.5321
2558 341.4886 1021.4440
2559 513.2769 1024.5392
2560 342.7068 1025.0986
2561 513.7318 1025.4490
2562 342.8237 1025.4493
2563 342.8239 1025.4499
2565 342.8242 1025.4508
2566 513.8083 1025.6020
2568 344.1682 1029.4828
2569 515.7490 1029.4834
2570 344.1687 1029.4843
2571 515.7503 1029.4860
2573 344.4837 1030.4293
2574 516.2860 1030.5574
2575 516.2866 1030.5586
2576 1031.6245 1030.6172
2577 516.3163 1030.6180
2578 516.3167 1030.6188
2579 516.7411 1031.4676
2580 517.2241 1032.4336
2582 518.7546 1035.4946
2583 346.1745 1035.5017
2584 518.7585 1035.5024
2585 519.2468 1036.4790
2586 521.2148 1040.4150
2587 348.1472 1041.4198
2588 521.7609 1041.5072
2596 349.4917 1045.4533
2597 523.7347 1045.4548
2598 349.8477 1046.5213
2599 350.1465 1047.4177
2600 524.7190 1047.4234
2601 524.7720 1047.5294
2602 524.8309 1047.6472
2603 525.2872 1048.5598
2604 350.8097 1049.4073
2606 526.7398 1051.4650
2607 527.3081 1052.6016
2608 527.7291 1053.4436
2609 352.1556 1053.4450
2611 527.8240 1053.6334
2612 353.4999 1057.4779
2613 529.7469 1057.4792
2614 530.2830 1058.5514
2617 532.7045 1063.3944
2618 355.5011 1063.4815
2620 532.7564 1063.4982
2624 356.8172 1067.4298
2625 535.2907 1068.5668
2626 535.3245 1068.6344
2627 535.7132 1069.4118
2628 357.4780 1069.4122
2631 535.8211 1069.6276
2633 537.2868 1072.5590
2634 358.8005 1073.3797
2635 358.8234 1073.4484
2636 538.2986 1074.5826
2637 538.2990 1074.5834
2638 1075.6520 1074.6447
2639 538.3302 1074.6458
2640 538.7232 1075.4318
2641 359.8600 1076.5582
2642 540.1855 1078.3564
2643 360.7964 1079.3674
2644 360.8270 1079.4592
2646 361.8428 1082.5066
2647 542.2815 1082.5484
2649 542.7962 1083.5778
2650 542.7966 1083.5786
2651 542.7985 1083.5824
2653 543.6982 1085.3818
2654 543.7446 1085.4746
2656 364.5236 1090.5490
2657 546.2822 1090.5498
2658 547.2880 1092.5614
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