MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2501–2600 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2175 704.3494 703.3421
2176 704.3628 703.3555
2177 352.6960 703.3774
2179 707.4241 706.4168
2180 709.3167 708.3094
2181 355.1863 708.3580
2184 357.6372 713.2598
2185 358.1809 714.3472
2186 358.1812 714.3478
2187 715.3557 714.3484
2188 358.1942 714.3738
2191 718.3182 717.3109
2194 360.1389 718.2632
2195 360.1784 718.3422
2198 360.7069 719.3992
2199 720.4066 719.3993
2201 360.7072 719.3998
2202 362.1914 722.3682
2203 723.4407 722.4334
2204 362.2243 722.4340
2205 363.0164 724.0182
2206 725.3954 724.3881
2207 363.2019 724.3892
2209 727.3757 726.3684
2210 364.1916 726.3686
2212 365.1577 728.3008
2215 365.6463 729.2780
2216 732.3314 731.3241
2217 732.3330 731.3257
2219 740.4672 739.4599
2220 371.1263 740.2380
2221 372.1938 742.3730
2223 743.4437 742.4364
2225 372.6891 743.3636
2226 372.6892 743.3638
2227 745.4222 744.4149
2228 373.2153 744.4160
2229 374.1355 746.2564
2230 748.4015 747.3942
2232 377.6846 753.3546
2233 756.3987 755.3914
2234 757.3630 756.3557
2235 379.7309 757.4472
2240 384.2065 766.3984
2241 767.4671 766.4598
2242 385.6449 769.2752
2243 385.6822 769.3498
2244 772.3671 771.3598
2245 386.7210 771.4274
2247 773.4171 772.4098
2252 391.7064 781.3982
2253 782.4066 781.3993
2254 392.2196 782.4246
2255 392.2199 782.4252
2257 395.2274 788.4402
2258 789.4490 788.4417
2259 789.4515 788.4442
2262 398.2037 794.3928
2263 399.6413 797.2680
2264 400.2169 798.4192
2266 402.2094 802.4042
2267 403.7305 805.4464
2269 405.2264 808.4382
2270 406.2196 810.4246
2271 811.4930 810.4857
2272 406.2502 810.4858
2273 407.2250 812.4354
2278 817.4462 816.4389
2284 412.7362 823.4578
2285 412.7550 823.4954
2286 413.6956 825.3766
2289 415.2331 828.4516
2290 832.4180 831.4107
2291 833.4751 832.4678
2292 417.2417 832.4688
2293 417.2482 832.4818
2294 417.2498 832.4850
2296 836.4631 835.4558
2297 421.7040 841.3934
2299 842.5126 841.5053
2302 423.6545 845.2944
2303 423.7232 845.4318
2304 428.2321 854.4496
2306 855.5191 854.5118
2307 428.2633 854.5120
2310 859.1013 858.0940
2311 863.9138 862.9065
2312 863.9143 862.9070
2313 432.7036 863.3926
2314 432.7572 863.4998
2315 432.7584 863.5022
2316 433.7381 865.4616
2318 435.7760 869.5374
2321 877.4993 876.4920
2322 439.2536 876.4926
2324 440.2336 878.4526
2326 441.2713 880.5280
2327 442.2304 882.4462
2328 884.4596 883.4523
2331 886.4069 885.3996
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