MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 4272)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2012 555.2634 554.2561
2013 556.2422 555.2349
2014 559.2166 558.2093
2015 559.2672 558.2599
2016 562.1403 561.1330
2017 567.2419 566.2346
2018 567.2447 566.2374
2019 568.2422 567.2349
2020 569.3058 568.2985
2021 569.3147 568.3074
2022 569.3418 568.3345
2023 571.8309 570.8236
2027 576.1566 575.1493
2028 576.2917 575.2844
2029 578.2615 577.2542
2030 581.2602 580.2529
2031 584.2294 583.2221
2032 588.5677 587.5604
2034 595.2762 594.2689
2035 596.6609 595.6536
2036 597.3114 596.3041
2037 597.3359 596.3286
2038 300.1895 598.3644
2039 301.1421 600.2696
2041 604.8735 603.8662
2042 605.2737 604.2664
2043 305.6882 609.3618
2044 613.3430 612.3357
2046 308.1667 614.3188
2047 310.6460 619.2774
2048 311.0990 620.1834
2051 312.6780 623.3414
2052 312.7139 623.4132
2053 313.6860 625.3574
2054 315.1697 628.3248
2055 630.3221 629.3148
2062 635.3882 634.3809
2063 319.6854 637.3562
2064 638.3644 637.3571
2065 319.6859 637.3572
2066 641.3397 640.3324
2067 321.2974 640.5802
2068 321.6833 641.3520
2077 322.1646 642.3146
2079 643.3629 642.3556
2084 651.3487 650.3414
2085 326.6756 651.3366
2086 652.4142 651.4069
2087 653.3467 652.3394
2088 654.3104 653.3031
2089 328.1649 654.3152
2090 656.3176 655.3103
2091 328.6625 655.3104
2092 329.1592 656.3038
2093 657.3688 656.3615
2094 329.6699 657.3252
2095 329.6699 657.3252
2096 329.6704 657.3262
2099 659.3904 658.3831
2106 664.4122 663.4049
2114 334.2243 666.4340
2115 335.6811 669.3476
2116 670.3550 669.3477
2118 336.1785 670.3424
2120 672.3471 671.3398
2121 336.6772 671.3398
2122 336.6783 671.3420
2123 336.6889 671.3632
2125 672.4067 671.3994
2126 337.1825 672.3504
2128 337.2007 672.3868
2129 337.6681 673.3216
2131 676.3424 675.3351
2135 339.1225 676.2304
2136 340.1796 678.3446
2137 679.4144 678.4071
2138 340.6925 679.3704
2139 680.3778 679.3705
2140 340.6934 679.3722
2141 341.6627 681.3108
2142 342.6561 683.2976
2145 343.6670 685.3194
2146 686.3375 685.3302
2147 343.6861 685.3576
2148 343.7011 685.3876
2149 344.1646 686.3146
2150 687.3359 686.3286
2152 688.3438 687.3365
2154 688.8622 687.8549
2155 345.1595 688.3044
2156 346.6730 691.3314
2161 346.7154 691.4162
2162 693.3480 692.3407
2164 696.4055 695.3982
2169 701.3944 700.3871
2170 351.6645 701.3144
2171 351.7007 701.3868
2172 703.2996 702.2923
2173 352.6600 703.3054
2174 704.3416 703.3343
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