MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2301–2400 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1896 453.2121 452.2048
1897 453.2124 452.2051
1898 453.2478 452.2405
1899 453.3444 452.3371
1900 454.2668 453.2595
1901 455.1605 454.1532
1902 455.1906 454.1833
1903 455.2276 454.2203
1904 455.2282 454.2209
1905 457.1395 456.1322
1906 457.1403 456.1330
1907 457.2775 456.2702
1908 458.2727 457.2654
1909 459.2823 458.2750
1910 461.2748 460.2675
1911 465.2117 464.2044
1912 465.2123 464.2050
1913 467.0645 466.0572
1914 467.0920 466.0847
1915 467.1611 466.1538
1916 467.2263 466.2190
1917 467.2267 466.2194
1918 469.2411 468.2338
1919 469.2414 468.2341
1920 469.2774 468.2701
1921 471.1547 470.1474
1922 471.2686 470.2613
1923 472.2531 471.2458
1924 472.2538 471.2465
1925 472.2904 471.2831
1926 472.3246 471.3173
1927 472.3256 471.3183
1928 473.1102 472.1029
1929 473.2050 472.1977
1930 473.2410 472.2337
1931 474.2688 473.2615
1932 474.2691 473.2618
1933 474.2693 473.2620
1934 474.2695 473.2622
1937 475.2900 474.2827
1938 475.3262 474.3189
1939 479.0012 477.9939
1940 479.1270 478.1197
1941 481.2637 480.2564
1942 487.1509 486.1436
1943 490.1194 489.1121
1944 493.0187 492.0114
1945 493.0189 492.0116
1946 493.0203 492.0130
1947 493.0204 492.0131
1948 493.0204 492.0131
1949 493.0205 492.0132
1950 493.0210 492.0137
1951 493.1164 492.1091
1952 496.2531 495.2458
1953 497.1716 496.1643
1954 497.5532 496.5459
1955 501.2372 500.2299
1956 501.2373 500.2300
1957 502.2265 501.2192
1958 502.2641 501.2568
1959 502.2647 501.2574
1961 503.3085 502.3012
1962 503.3086 502.3013
1963 511.3037 510.2964
1964 513.2795 512.2722
1965 513.2800 512.2727
1966 514.2853 513.2780
1967 515.2642 514.2569
1968 515.3306 514.3233
1970 519.3170 518.3097
1971 520.3354 519.3281
1972 524.2841 523.2768
1973 524.2842 523.2769
1974 525.0486 524.0413
1975 525.0490 524.0417
1976 525.2335 524.2262
1977 525.2907 524.2834
1980 529.2751 528.2678
1981 529.2751 528.2678
1982 529.2756 528.2683
1984 529.2760 528.2687
1985 529.2761 528.2688
1988 529.3480 528.3407
1989 530.3303 529.3230
1990 531.1163 530.1090
1993 536.3107 535.3034
1994 537.2354 536.2281
1995 537.2358 536.2285
1996 537.2366 536.2293
1997 538.2302 537.2229
1998 538.2333 537.2260
1999 539.3301 538.3228
2004 545.2708 544.2635
2005 546.2549 545.2476
2007 547.9811 546.9738
2008 550.2674 549.2601
2009 552.2797 551.2724
2010 552.2808 551.2735
2011 553.2849 552.2776
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