MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Unassigned peptides, 2201–2300 (out of 4272)

Page: Previous 1 …18 19 20 21 22 23 24 25 26 27 28 … 43 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1796 425.2167 424.2094
1797 425.2168 424.2095
1798 425.2168 424.2095
1799 425.2170 424.2097
1800 425.2173 424.2100
1801 425.2174 424.2101
1802 425.2356 424.2283
1803 425.2356 424.2283
1804 425.2357 424.2284
1805 425.2357 424.2284
1806 425.2358 424.2285
1807 425.2358 424.2285
1808 426.0650 425.0577
1809 427.0929 426.0856
1810 427.1291 426.1218
1811 427.1292 426.1219
1812 427.1668 426.1595
1813 427.1956 426.1883
1814 427.1958 426.1885
1815 427.1960 426.1887
1816 427.2310 426.2237
1817 427.2562 426.2489
1818 429.1083 428.1010
1819 429.1458 428.1385
1820 429.1814 428.1741
1821 429.2108 428.2035
1822 432.2822 431.2749
1823 433.1854 432.1781
1824 434.0043 432.9970
1825 435.2004 434.1931
1826 435.2011 434.1938
1827 435.2012 434.1939
1828 435.2012 434.1939
1829 435.2012 434.1939
1830 435.2013 434.1940
1831 435.2018 434.1945
1832 435.2019 434.1946
1833 436.0428 435.0355
1834 437.1798 436.1725
1835 437.1809 436.1736
1836 437.1811 436.1738
1837 437.2155 436.2082
1838 437.2157 436.2084
1839 437.2159 436.2086
1840 437.2160 436.2087
1841 437.2162 436.2089
1842 437.2165 436.2092
1843 437.2168 436.2095
1844 437.2169 436.2096
1845 437.2169 436.2096
1846 437.2171 436.2098
1847 437.2174 436.2101
1848 437.2379 436.2306
1849 437.2519 436.2446
1850 437.2521 436.2448
1851 437.2523 436.2450
1852 438.1661 437.1588
1853 438.2531 437.2458
1854 439.1952 438.1879
1855 439.1960 438.1887
1856 439.1967 438.1894
1857 439.1969 438.1896
1858 439.1973 438.1900
1859 439.2134 438.2061
1860 439.2141 438.2068
1861 439.2141 438.2068
1862 439.2141 438.2068
1863 439.2149 438.2076
1864 439.2149 438.2076
1865 439.2151 438.2078
1866 439.2152 438.2079
1867 439.2152 438.2079
1868 439.2153 438.2080
1869 439.2309 438.2236
1870 439.2315 438.2242
1871 439.2317 438.2244
1872 439.2318 438.2245
1873 439.2325 438.2252
1874 439.2325 438.2252
1875 439.2327 438.2254
1876 439.2332 438.2259
1877 441.2307 440.2234
1878 442.2424 441.2351
1879 442.2428 441.2355
1880 443.2466 442.2393
1881 443.2467 442.2394
1882 443.2471 442.2398
1883 444.1100 443.1027
1884 449.2174 448.2101
1885 449.7263 448.7190
1886 451.1957 450.1884
1887 451.1961 450.1888
1888 451.1962 450.1889
1889 453.2100 452.2027
1890 453.2108 452.2035
1891 453.2109 452.2036
1892 453.2114 452.2041
1893 453.2116 452.2043
1894 453.2118 452.2045
1895 453.2119 452.2046
Page: Previous 1 …18 19 20 21 22 23 24 25 26 27 28 … 43 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/