MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2101–2200 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1696 415.1297 414.1224
1697 415.2554 414.2481
1698 416.2141 415.2068
1699 416.2625 415.2552
1700 416.2627 415.2554
1701 416.2628 415.2555
1702 416.2631 415.2558
1703 416.2633 415.2560
1704 416.2635 415.2562
1705 416.3752 415.3679
1706 416.3756 415.3683
1707 417.1455 416.1382
1708 417.2469 416.2396
1709 417.2484 416.2411
1710 418.0322 417.0249
1711 418.2059 417.1986
1712 419.1697 418.1624
1713 421.0579 420.0506
1714 421.1473 420.1400
1715 421.1842 420.1769
1716 421.1845 420.1772
1717 421.1849 420.1776
1718 421.1849 420.1776
1719 421.1854 420.1781
1720 421.1856 420.1783
1721 421.1856 420.1783
1722 421.1860 420.1787
1723 421.1861 420.1788
1724 421.2029 420.1956
1725 421.2031 420.1958
1726 421.2037 420.1964
1727 421.2039 420.1966
1728 421.2044 420.1971
1729 421.2044 420.1971
1730 421.2044 420.1971
1731 421.2045 420.1972
1732 421.2055 420.1982
1733 421.2212 420.2139
1734 421.2213 420.2140
1735 421.2219 420.2146
1736 421.2219 420.2146
1737 421.2221 420.2148
1738 421.2224 420.2151
1739 421.2225 420.2152
1740 421.2227 420.2154
1741 421.2228 420.2155
1742 423.1358 422.1285
1743 423.1637 422.1564
1744 423.1640 422.1567
1745 423.1650 422.1577
1746 423.1651 422.1578
1747 423.1994 422.1921
1748 423.1999 422.1926
1749 423.1999 422.1926
1750 423.2000 422.1927
1751 423.2001 422.1928
1752 423.2003 422.1930
1753 423.2004 422.1931
1754 423.2004 422.1931
1755 423.2006 422.1933
1756 423.2006 422.1933
1757 423.2006 422.1933
1758 423.2010 422.1937
1759 423.2010 422.1937
1760 423.2010 422.1937
1761 423.2011 422.1938
1762 423.2012 422.1939
1763 423.2012 422.1939
1764 423.2018 422.1945
1765 423.2019 422.1946
1766 423.2020 422.1947
1767 423.2186 422.2113
1768 423.2186 422.2113
1769 423.2191 422.2118
1770 423.2194 422.2121
1771 423.2198 422.2125
1772 423.2198 422.2125
1773 423.2199 422.2126
1774 423.2201 422.2128
1775 423.2201 422.2128
1776 423.2201 422.2128
1777 423.2202 422.2129
1778 423.2203 422.2130
1779 423.2261 422.2188
1780 423.2262 422.2189
1781 424.0497 423.0424
1782 425.1512 424.1439
1783 425.1797 424.1724
1784 425.1797 424.1724
1785 425.1800 424.1727
1786 425.1801 424.1728
1787 425.1802 424.1729
1788 425.1803 424.1730
1789 425.1806 424.1733
1790 425.2152 424.2079
1791 425.2161 424.2088
1792 425.2162 424.2089
1793 425.2163 424.2090
1794 425.2164 424.2091
1795 425.2165 424.2092
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