MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2001–2100 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1596 405.2273 404.2200
1597 407.1319 406.1246
1598 407.1679 406.1606
1599 407.1686 406.1613
1600 407.1687 406.1614
1601 407.1687 406.1614
1602 407.1691 406.1618
1603 407.1694 406.1621
1604 407.1694 406.1621
1605 407.1697 406.1624
1606 407.1699 406.1626
1607 407.2049 406.1976
1608 407.2054 406.1981
1609 407.2059 406.1986
1610 407.2060 406.1987
1611 407.2061 406.1988
1612 407.2061 406.1988
1613 407.2063 406.1990
1614 407.2063 406.1990
1615 407.2065 406.1992
1616 407.2069 406.1996
1617 407.2069 406.1996
1618 407.2072 406.1999
1619 407.2072 406.1999
1620 407.2074 406.2001
1621 407.2083 406.2010
1622 407.2242 406.2169
1623 407.2248 406.2175
1624 407.2250 406.2177
1625 407.2252 406.2179
1626 407.2252 406.2179
1627 407.2253 406.2180
1628 407.2253 406.2180
1629 407.2284 406.2211
1630 409.1481 408.1408
1631 409.1490 408.1417
1632 409.1491 408.1418
1633 409.1626 408.1553
1634 409.1645 408.1572
1635 409.1842 408.1769
1636 409.1844 408.1771
1637 409.1845 408.1772
1638 409.1848 408.1775
1639 409.1851 408.1778
1640 409.1851 408.1778
1641 409.1852 408.1779
1642 409.1853 408.1780
1643 409.1854 408.1781
1644 409.1854 408.1781
1645 409.1855 408.1782
1646 409.1856 408.1783
1647 409.1858 408.1785
1648 409.1858 408.1785
1649 409.1860 408.1787
1650 409.1865 408.1792
1651 409.2204 408.2131
1652 409.2207 408.2134
1653 409.2217 408.2144
1654 409.2221 408.2148
1655 409.2224 408.2151
1656 409.2248 408.2175
1657 409.2393 408.2320
1658 409.2397 408.2324
1659 409.2405 408.2332
1660 409.2406 408.2333
1661 409.2406 408.2333
1662 409.2407 408.2334
1663 409.2408 408.2335
1664 410.2437 409.2364
1665 410.2441 409.2368
1666 411.0980 410.0907
1667 411.1632 410.1559
1668 411.1641 410.1568
1669 411.1642 410.1569
1670 411.1643 410.1570
1671 411.1645 410.1572
1672 411.1645 410.1572
1673 411.1993 410.1920
1674 411.1999 410.1926
1675 411.2000 410.1927
1676 411.2000 410.1927
1677 411.2000 410.1927
1678 411.2003 410.1930
1679 411.2006 410.1933
1680 411.2008 410.1935
1681 411.2010 410.1937
1682 413.1140 412.1067
1683 413.1796 412.1723
1684 413.1801 412.1728
1685 413.1802 412.1729
1686 413.1804 412.1731
1687 413.1804 412.1731
1688 413.1808 412.1735
1689 413.1815 412.1742
1690 413.2164 412.2091
1691 413.2676 412.2603
1692 414.2468 413.2395
1693 415.0938 414.0865
1694 415.1287 414.1214
1695 415.1294 414.1221
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