MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1496 393.2995 392.2922
1497 393.2995 392.2922
1498 393.2995 392.2922
1499 393.2996 392.2923
1500 393.2996 392.2923
1501 393.2996 392.2923
1502 393.2996 392.2923
1503 393.2997 392.2924
1504 393.2998 392.2925
1505 393.2999 392.2926
1506 393.2999 392.2926
1507 393.3000 392.2927
1508 393.3000 392.2927
1509 393.3001 392.2928
1510 393.3001 392.2928
1511 393.3002 392.2929
1512 393.3002 392.2929
1513 395.0776 394.0703
1514 395.1683 394.1610
1515 395.1687 394.1614
1516 395.1689 394.1616
1517 395.1689 394.1616
1518 395.1690 394.1617
1519 395.1690 394.1617
1520 395.1690 394.1617
1521 395.1692 394.1619
1522 395.1693 394.1620
1523 395.1696 394.1623
1524 395.1696 394.1623
1525 395.1698 394.1625
1526 395.1698 394.1625
1527 395.1703 394.1630
1528 395.1703 394.1630
1529 395.1704 394.1631
1530 395.1704 394.1631
1531 395.2046 394.1973
1532 395.2048 394.1975
1533 395.2054 394.1981
1534 395.2061 394.1988
1535 395.2061 394.1988
1536 395.2061 394.1988
1537 395.2063 394.1990
1538 395.2068 394.1995
1539 395.2068 394.1995
1540 395.2072 394.1999
1541 396.0531 395.0458
1542 396.9600 395.9527
1543 397.0826 396.0753
1544 397.1192 396.1119
1545 397.1291 396.1218
1546 397.1483 396.1410
1547 397.1497 396.1424
1548 397.1843 396.1770
1549 397.1844 396.1771
1550 397.1844 396.1771
1551 397.1846 396.1773
1552 397.1847 396.1774
1553 397.1848 396.1775
1554 397.1850 396.1777
1555 397.1852 396.1779
1556 397.1853 396.1780
1557 397.1854 396.1781
1558 397.1854 396.1781
1559 397.1855 396.1782
1560 397.1855 396.1782
1561 397.1857 396.1784
1562 397.1857 396.1784
1563 397.1861 396.1788
1564 397.2201 396.2128
1565 397.2202 396.2129
1566 397.2210 396.2137
1567 397.2817 396.2744
1568 399.0977 398.0904
1569 399.0979 398.0906
1570 399.1531 398.1458
1571 399.2007 398.1934
1572 399.2011 398.1938
1573 399.2012 398.1939
1574 399.2360 398.2287
1575 400.2311 399.2238
1576 401.1141 400.1068
1577 401.2876 400.2803
1578 401.2884 400.2811
1579 402.0540 401.0467
1580 402.2477 401.2404
1581 403.1736 402.1663
1582 403.9776 402.9703
1583 404.2662 403.2589
1584 405.1897 404.1824
1585 405.1902 404.1829
1586 405.1906 404.1833
1587 405.1907 404.1834
1588 405.1909 404.1836
1589 405.1909 404.1836
1590 405.1912 404.1839
1591 405.1913 404.1840
1592 405.1926 404.1853
1593 405.2148 404.2075
1594 405.2267 404.2194
1595 405.2273 404.2200
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