MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1249__2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues) |
| Timestamp | : | 23 Jun 2025 at 17:45:54 GMT |
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the select summary.
| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,602 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 2144 |
342.6855 | 683.3564 | 683.3463 | 14.9 | 1 | 7 | 0.25 | 1Score > 13 indicates identity |
HESRR |
| 3020 |
669.3570 | 1336.6994 | 1336.7099 | -7.80 | 1 | 7 | 0.89 | 1Score > 19 indicates identity |
SPSAQKLEALHR + Deamidated (NQ) |
| 3176 |
702.8515 | 1403.6884 | 1403.6867 | 1.24 | 0 | 7 | 0.96 | 1Score > 19 indicates identity |
GISDLAQHYLMR + Deamidated (NQ) |
| 3791 |
471.4737 | 1881.8657 | 1881.8639 | 0.97 | 1 | 7 | 0.89 | 1Score > 19 indicates identity |
ADCEQLAELRSHAPER + Deamidated (NQ) |
| 2454 |
479.2631 | 956.5116 | 956.5291 | -18.2 | 0 | 6 | 1 | 1Score > 19 indicates identity |
QDILEAIR |
| 3575 |
438.7355 | 1750.9129 | 1750.9101 | 1.61 | 0 | 6 | 0.76 | 1Score > 18 indicates identity |
LAEPAPTGEQLQNILR + 2 Deamidated (NQ) |
| 2366 |
457.2010 | 912.3874 | 912.3937 | -6.84 | 0 | 6 | 0.23 | 1Score > 13 indicates identity |
DQLNHQR + 3 Deamidated (NQ) |
| 4239 |
827.7393 | 2480.1961 | 2480.1828 | 5.36 | 1 | 6 | 0.94 | 1Score > 19 indicates identity |
AMRLLVPPAWQNNPDMDPELR + 2 Deamidated (NQ); Oxidation (M) |
| 4106 |
534.0483 | 2132.1641 | 2132.1266 | 17.6 | 1 | 6 | 0.41 | 1Score > 15 indicates identity |
ESIQIFHNPRVLVEPEPK + Deamidated (NQ) |
| 3140 |
347.9352 | 1387.7117 | 1387.6983 | 9.63 | 0 | 6 | 1.1 | 1Score > 19 indicates identity |
QQNVPVFLLGDR + 3 Deamidated (NQ) |
| 2104 |
332.6746 | 663.3346 | 663.3374 | -4.11 | 0 | 6 | 1.2 | 1Score > 20 indicates identity |
AALSMR + Oxidation (M) |
| 2200 |
720.4066 | 719.3993 | 719.3966 | 3.78 | 0 | 6 | 0.69 | 1Score > 17 indicates identity |
ALPTYR |
| 3039 |
337.4402 | 1345.7317 | 1345.7282 | 2.62 | 0 | 6 | 0.67 | 1Score > 17 indicates identity |
GEYLPIVELWK |
| 3579 |
438.7370 | 1750.9189 | 1750.9254 | -3.70 | 1 | 6 | 0.77 | 1Score > 17 indicates identity |
AFKDLGFDSLAAVELR |
| 2828 |
621.2905 | 1240.5664 | 1240.5870 | -16.5 | 1 | 6 | 0.57 | 1Score > 16 indicates identity |
NSYMGRLINR + 2 Deamidated (NQ); Oxidation (M) |
| 2867 |
629.3173 | 1256.6200 | 1256.6010 | 15.1 | 0 | 6 | 0.81 | 1Score > 18 indicates identity |
LAEHGATEHHR |
| 3200 |
710.8478 | 1419.6810 | 1419.6590 | 15.6 | 1 | 6 | 1 | 1Score > 19 indicates identity |
SVSRLGNSEQQGR + 3 Deamidated (NQ) |
| 4069 |
518.7434 | 2070.9445 | 2070.9106 | 16.4 | 0 | 6 | 0.86 | 1Score > 18 indicates identity |
CVVVVDHYDWEDDAPPR |
| 2132 |
338.6754 | 675.3362 | 675.3261 | 15.0 | 0 | 6 | 1.1 | 1Score > 19 indicates identity |
AMAELAA |
| 3979 |
670.6836 | 2009.0290 | 2009.0079 | 10.5 | 1 | 6 | 1.2 | 1Score > 19 indicates identity |
RWLESQGVDVANGSNHLK |
| 2279 |
411.7290 | 821.4434 | 821.4508 | -8.90 | 1 | 6 | 0.7 | 1Score > 17 indicates identity |
QRLSYR |
| 3116 |
345.4383 | 1377.7241 | 1377.7252 | -0.79 | 0 | 6 | 0.94 | 1Score > 18 indicates identity |
KPDHQLAALQEK + Deamidated (NQ) |
| 3501 |
857.9945 | 1713.9744 | 1713.9625 | 6.98 | 1 | 6 | 0.3 | 1Score > 13 indicates identity |
GVELLSTGGTARLLAEK |
| 2282 |
822.4549 | 821.4476 | 821.4508 | -3.82 | 1 | 6 | 0.45 | 1Score > 15 indicates identity |
QRLSYR |
| 2982 |
439.2336 | 1314.6790 | 1314.6932 | -10.8 | 0 | 6 | 1.2 | 1Score > 19 indicates identity |
AQNHLYTVLEK |
| 2467 |
324.1748 | 969.5026 | 969.4920 | 10.9 | 1 | 6 | 0.64 | 1Score > 16 indicates identity |
IRDYFQK + Deamidated (NQ) |
| 3150 |
464.9209 | 1391.7409 | 1391.7482 | -5.30 | 1 | 6 | 0.83 | 1Score > 17 indicates identity |
QLALFKEMQGVK + Deamidated (NQ) |
| 2401 |
930.5268 | 929.5195 | 929.5182 | 1.45 | 0 | 6 | 1 | 1Score > 18 indicates identity |
IITQADLR + Deamidated (NQ) |
| 2545 |
338.1864 | 1011.5374 | 1011.5284 | 8.92 | 1 | 6 | 0.43 | 1Score > 14 indicates identity |
MLQKHAER |
| 2619 |
532.7538 | 1063.4930 | 1063.4968 | -3.51 | 1 | 6 | 0.75 | 1Score > 17 indicates identity |
TAIDMDKNR + Deamidated (NQ) |
| 3896 |
980.0093 | 1958.0040 | 1958.0295 | -13.0 | 1 | 5 | 1.2 | 1Score > 19 indicates identity |
NLTILDMFGPPKTNAGIR + Deamidated (NQ) |
| 2325 |
440.2341 | 878.4536 | 878.4610 | -8.37 | 1 | 5 | 0.98 | 1Score > 18 indicates identity |
PSTRYQK |
| 4531 |
927.7491 | 5560.4509 | 5560.5454 | -17.0 | 1 | 5 | 0.29 | 1Score > 13 indicates identity |
APGSSSSGANGDGSLAQSQTGAVVRATNAASDFTLLELNTAANPAYNLFWAGWDR + 6 Deamidated (NQ) |
| 2564 |
513.7325 | 1025.4504 | 1025.4600 | -9.32 | 0 | 5 | 0.45 | 1Score > 14 indicates identity |
ISQYACQR + Deamidated (NQ) |
| 4415 |
978.4999 | 2932.4779 | 2932.4964 | -6.31 | 1 | 5 | 1.1 | 1Score > 18 indicates identity |
FNGKPASGLGVKLASGANEMATAELVLNR + 2 Deamidated (NQ); Oxidation (M) |
| 3129 |
461.9169 | 1382.7289 | 1382.7228 | 4.42 | 1 | 5 | 1 | 1Score > 18 indicates identity |
LAMQSVRSLFSK + Deamidated (NQ); Oxidation (M) |
| 2797 |
613.2822 | 1224.5498 | 1224.5371 | 10.4 | 0 | 5 | 0.59 | 1Score > 16 indicates identity |
TDQYGGSVENR |
| 3118 |
460.2490 | 1377.7252 | 1377.7041 | 15.3 | 0 | 5 | 1.1 | 1Score > 18 indicates identity |
FPYVNANVIDAR |
| 2853 |
628.3252 | 1254.6358 | 1254.6431 | -5.75 | 1 | 5 | 0.94 | 1Score > 17 indicates identity |
FMTFISPEKR |
| 4221 |
488.8435 | 2439.1811 | 2439.2250 | -18.0 | 1 | 5 | 1 | 1Score > 20 indicates identity Score > 18 indicates homology |
SNIGHPQGAAGVAGVIKMVMALQR + 3 Deamidated (NQ); 2 Oxidation (M) |
| 2295 |
418.2053 | 834.3960 | 834.4018 | -6.85 | 1 | 5 | 0.83 | 1Score > 17 indicates identity |
SAMDKGAR |
| 2418 |
471.7764 | 941.5382 | 941.5406 | -2.52 | 1 | 5 | 0.67 | 1Score > 16 indicates identity |
IALAENRR |
| 2733 |
393.5224 | 1177.5454 | 1177.5615 | -13.7 | 0 | 5 | 1.1 | 1Score > 18 indicates identity |
VLQEQEFER + Deamidated (NQ) |
| 2515 |
331.8262 | 992.4568 | 992.4597 | -2.94 | 0 | 5 | 0.7 | 1Score > 16 indicates identity |
MATTVEADR |
| 3034 |
671.8710 | 1341.7274 | 1341.7153 | 9.04 | 0 | 5 | 1.6 | 1Score > 19 indicates identity |
LLADPHLGHVNR + Deamidated (NQ) |
| 3877 |
488.2453 | 1948.9521 | 1948.9676 | -7.97 | 0 | 5 | 0.96 | 1Score > 20 indicates identity Score > 17 indicates homology |
MLAQSLQALEQDGFLNR + Oxidation (M) |
| 3248 |
478.5898 | 1432.7476 | 1432.7424 | 3.58 | 0 | 5 | 1.5 | 1Score > 19 indicates identity |
IWFIPAMPETTK |
| 2192 |
718.3281 | 717.3208 | 717.3293 | -11.8 | 0 | 5 | 0.62 | 1Score > 15 indicates identity |
VEGEER |
| 4196 |
803.7543 | 2408.2411 | 2408.2547 | -5.65 | 1 | 5 | 1.4 | 1Score > 19 indicates identity |
NLKLALTAGIGSDHVDLQSAIDR + 2 Deamidated (NQ) |
| 3142 |
694.8682 | 1387.7218 | 1387.6943 | 19.9 | 1 | 5 | 1.5 | 1Score > 19 indicates identity |
AIDSSPDKANLTR + Deamidated (NQ) |
| 2525 |
502.2526 | 1002.4906 | 1002.4730 | 17.6 | 0 | 5 | 1.5 | 1Score > 19 indicates identity |
NESAGEQLR |
| 3478 |
565.9914 | 1694.9524 | 1694.9679 | -9.14 | 0 | 5 | 0.51 | 1Score > 14 indicates identity |
LLANRPESALAVTLAR + Deamidated (NQ) |
| 3809 |
633.6198 | 1897.8376 | 1897.8186 | 10.0 | 0 | 5 | 0.86 | 1Score > 17 indicates identity |
SCAFLIADGVMPSNENR + 2 Deamidated (NQ); Oxidation (M) |
| 4530 |
1112.8981 | 5559.4541 | 5559.5031 | -8.80 | 1 | 5 | 0.34 | 1Score > 13 indicates identity |
AFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFNAETVKMFTTSSK + 5 Deamidated (NQ); 3 Oxidation (M) |
| 3177 |
351.9294 | 1403.6885 | 1403.7005 | -8.52 | 1 | 5 | 1.5 | 1Score > 19 indicates identity |
RSETETVADALGR |
| 2281 |
822.4536 | 821.4463 | 821.4508 | -5.40 | 1 | 5 | 0.58 | 1Score > 15 indicates identity |
QRLSYR |
| 4380 |
924.1334 | 2769.3784 | 2769.4218 | -15.7 | 0 | 5 | 1.6 | 1Score > 19 indicates identity |
IMDLQSAQQLGEIAAAVGLAQNLGALR + 3 Deamidated (NQ); Oxidation (M) |
| 2887 |
636.3586 | 1270.7026 | 1270.6881 | 11.5 | 1 | 5 | 1.2 | 1Score > 18 indicates identity |
DGKINLLDLNR + Deamidated (NQ) |
| 3868 |
646.9586 | 1937.8540 | 1937.8789 | -12.9 | 0 | 5 | 0.7 | 1Score > 16 indicates identity |
LPSTCFAEENGSIVNSGR + Deamidated (NQ) |
| 3414 |
818.4063 | 1634.7980 | 1634.7934 | 2.86 | 1 | 4 | 1.8 | 1Score > 19 indicates identity |
SANMIDRDTLDALGK + Oxidation (M) |
| 4211 |
607.0618 | 2424.2181 | 2424.2145 | 1.47 | 1 | 4 | 1.8 | 1Score > 19 indicates identity |
SHGAANQRAFAVAIEQAVQAVQR + 3 Deamidated (NQ) |
| 2712 |
577.2811 | 1152.5476 | 1152.5267 | 18.2 | 1 | 4 | 1.2 | 1Score > 18 indicates identity |
MLQRMDSEK + Oxidation (M) |
| 2684 |
561.8037 | 1121.5928 | 1121.5829 | 8.86 | 1 | 4 | 1.4 | 1Score > 18 indicates identity |
GSAFTQEVKR |
| 3580 |
584.6470 | 1750.9192 | 1750.9002 | 10.8 | 0 | 4 | 1.2 | 1Score > 17 indicates identity |
VNNFQVSAASTPFLTR |
| 3623 |
590.6443 | 1768.9111 | 1768.9206 | -5.41 | 1 | 4 | 1.5 | 1Score > 18 indicates identity |
ALDVATNVLQRNPNIK + 4 Deamidated (NQ) |
| 2419 |
471.7767 | 941.5388 | 941.5406 | -1.88 | 1 | 4 | 0.79 | 1Score > 16 indicates identity |
IALAENRR |
| 4340 |
658.3148 | 2629.2301 | 2629.2368 | -2.56 | 1 | 4 | 1.6 | 1Score > 19 indicates identity |
TTEQSWQQQTRTSQAAPVQAQPR + 3 Deamidated (NQ) |
| 2870 |
420.8715 | 1259.5927 | 1259.5854 | 5.76 | 1 | 4 | 0.99 | 1Score > 17 indicates identity |
GDASGDALNRQR + Deamidated (NQ) |
| 3980 |
670.6840 | 2009.0302 | 2008.9966 | 16.7 | 0 | 4 | 1.7 | 1Score > 19 indicates identity |
ASNGLNIHLNHYESLLGR + 2 Deamidated (NQ) |
| 2964 |
327.1675 | 1304.6409 | 1304.6361 | 3.69 | 0 | 4 | 1.5 | 1Score > 18 indicates identity |
WQSTSVNDVLR + Deamidated (NQ) |
| 3041 |
337.4408 | 1345.7341 | 1345.7606 | -19.7 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
VYPLTVGDKVQK |
| 3519 |
864.4838 | 1726.9530 | 1726.9577 | -2.70 | 1 | 4 | 1 | 1Score > 17 indicates identity |
LNALEGKLQTGEISVR |
| 2824 |
621.2666 | 1240.5186 | 1240.5207 | -1.68 | 0 | 4 | 0.4 | 1Score > 13 indicates identity |
TEQQYENATR + 2 Deamidated (NQ) |
| 2881 |
634.3641 | 1266.7136 | 1266.6932 | 16.1 | 0 | 4 | 0.46 | 1Score > 13 indicates identity |
TPVALLDAPASGR |
| 2781 |
606.8049 | 1211.5952 | 1211.6186 | -19.3 | 0 | 4 | 1.5 | 1Score > 18 indicates identity |
QLGVSYFLER + Deamidated (NQ) |
| 3601 |
878.9440 | 1755.8734 | 1755.9050 | -18.0 | 1 | 4 | 1.8 | 1Score > 19 indicates identity |
VQQGRLVVNGASMPQR + Deamidated (NQ); Oxidation (M) |
| 3044 |
337.4412 | 1345.7357 | 1345.7136 | 16.4 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
MSELIVSRQQR |
| 2287 |
414.2058 | 826.3970 | 826.3933 | 4.54 | 1 | 4 | 0.92 | 1Score > 16 indicates identity |
EKHQQR + 2 Deamidated (NQ) |
| 2343 |
893.4508 | 892.4435 | 892.4403 | 3.66 | 1 | 4 | 1.3 | 1Score > 18 indicates identity |
AAFEDKGR |
| 2741 |
395.8608 | 1184.5606 | 1184.5397 | 17.6 | 0 | 4 | 1.2 | 1Score > 17 indicates identity |
HCVWEEVAR |
| 3125 |
345.9425 | 1379.7409 | 1379.7483 | -5.35 | 0 | 4 | 0.86 | 1Score > 16 indicates identity |
IIFCGTLTAGSLK |
| 3872 |
485.9830 | 1939.9029 | 1939.9244 | -11.1 | 0 | 4 | 1.2 | 1Score > 17 indicates identity |
HLQQMQHSLGMTVGTVR + 2 Deamidated (NQ); Oxidation (M) |
| 2178 |
354.1964 | 706.3782 | 706.3796 | -1.88 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
LRSGMK + Oxidation (M) |
| 3611 |
881.4874 | 1760.9602 | 1760.9308 | 16.7 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
LLQDADVFITNVREK + Deamidated (NQ) |
| 4076 |
695.0237 | 2082.0493 | 2082.0527 | -1.66 | 1 | 4 | 1.9 | 1Score > 19 indicates identity |
MNSIEQALKIAEQHNISR + Deamidated (NQ) |
| 2661 |
548.7223 | 1095.4300 | 1095.4469 | -15.4 | 1 | 4 | 0.41 | 1Score > 13 indicates identity |
DFEDKDDGR |
| 4361 |
895.4604 | 2683.3594 | 2683.3470 | 4.60 | 0 | 4 | 1.6 | 1Score > 18 indicates identity |
ALAQAMHQAGKPLGFMCIAPAMLPK + 2 Oxidation (M) |
| 4251 |
835.0648 | 2502.1726 | 2502.2060 | -13.4 | 0 | 4 | 1.7 | 1Score > 19 indicates identity |
SIIPSGYGVNPEGPTQECLSALGR + Deamidated (NQ) |
| 2280 |
822.4517 | 821.4444 | 821.4508 | -7.71 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
QRLSYR |
| 2358 |
302.8324 | 905.4754 | 905.4607 | 16.2 | 0 | 4 | 2 | 1Score > 19 indicates identity |
QAGKPYDK |
| 2002 |
543.3621 | 542.3548 | 542.3540 | 1.51 | 0 | 4 | 1.8 | 1Score > 19 indicates identity |
AAIIR |
| 2317 |
435.7578 | 869.5010 | 869.5083 | -8.31 | 1 | 4 | 0.64 | 1Score > 14 indicates identity |
ERLLSPR |
| 4014 |
509.0045 | 2031.9889 | 2031.9709 | 8.88 | 1 | 4 | 0.54 | 1Score > 20 indicates identity Score > 13 indicates homology |
AEPTSGGGQLSTAQKQNISR + 3 Deamidated (NQ) |
| 3156 |
465.5809 | 1393.7209 | 1393.7388 | -12.8 | 1 | 4 | 1.5 | 1Score > 18 indicates identity |
LGERSCAYVVLK |
| 3447 |
555.9594 | 1664.8564 | 1664.8774 | -12.6 | 1 | 4 | 1.9 | 1Score > 19 indicates identity |
AQVAIFKEIFDQVR + 2 Deamidated (NQ) |
| 2372 |
459.7207 | 917.4268 | 917.4202 | 7.20 | 1 | 3 | 1.3 | 1Score > 17 indicates identity |
EANEGDKR |
| 4070 |
519.7497 | 2074.9697 | 2075.0106 | -19.7 | 1 | 3 | 1.7 | 1Score > 18 indicates identity |
RVGETAWAQDTIADCLLR + Deamidated (NQ) |
| 2390 |
310.1582 | 927.4528 | 927.4661 | -14.4 | 0 | 3 | 0.8 | 1Score > 15 indicates identity |
GLLPENER + Deamidated (NQ) |
| 3991 |
673.6527 | 2017.9363 | 2017.9626 | -13.1 | 0 | 3 | 1.6 | 1Score > 18 indicates identity |
IDVSQVDVSMNGIELQDR + Deamidated (NQ) |
| 3999 |
675.3585 | 2023.0537 | 2023.0442 | 4.67 | 1 | 3 | 1.5 | 1Score > 18 indicates identity |
VMAGLGIAVVSTSKGVMTDR + 2 Oxidation (M) |
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