MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1396 383.2063 382.1990
1397 383.2065 382.1992
1398 383.2067 382.1994
1399 383.2067 382.1994
1400 383.2068 382.1995
1401 383.2068 382.1995
1402 383.2068 382.1995
1403 384.0514 383.0441
1404 385.0215 384.0142
1405 385.0826 384.0753
1406 385.1186 384.1113
1407 385.1198 384.1125
1408 385.1360 384.1287
1409 385.1845 384.1772
1410 385.1848 384.1775
1411 385.1853 384.1780
1412 385.1854 384.1781
1413 385.1855 384.1782
1414 385.1857 384.1784
1415 385.1860 384.1787
1416 385.1860 384.1787
1417 385.2215 384.2142
1418 385.2450 384.2377
1419 385.2462 384.2389
1420 385.2572 384.2499
1421 385.2574 384.2501
1422 385.2577 384.2504
1423 386.2407 385.2334
1424 387.0975 386.0902
1425 387.1356 386.1283
1426 387.2000 386.1927
1427 387.2000 386.1927
1428 387.2001 386.1928
1429 387.2005 386.1932
1430 387.2011 386.1938
1431 387.2012 386.1939
1432 387.2014 386.1941
1433 387.2042 386.1969
1434 388.2314 387.2241
1435 389.0521 388.0448
1436 389.1595 388.1522
1437 389.1962 388.1889
1438 391.1393 390.1320
1439 391.1741 390.1668
1440 391.1742 390.1669
1441 391.1744 390.1671
1442 391.1745 390.1672
1443 391.1751 390.1678
1444 391.1752 390.1679
1445 391.1752 390.1679
1446 391.1754 390.1681
1447 391.2110 390.2037
1448 391.2117 390.2044
1449 391.2118 390.2045
1450 391.2118 390.2045
1451 391.2119 390.2046
1452 391.2862 390.2789
1453 391.2863 390.2790
1454 393.1519 392.1446
1455 393.1524 392.1451
1456 393.1526 392.1453
1457 393.1526 392.1453
1458 393.1530 392.1457
1459 393.1531 392.1458
1460 393.1532 392.1459
1461 393.1537 392.1464
1462 393.1543 392.1470
1463 393.1545 392.1472
1464 393.1545 392.1472
1465 393.1892 392.1819
1466 393.1895 392.1822
1467 393.1895 392.1822
1468 393.1897 392.1824
1469 393.1902 392.1829
1470 393.1902 392.1829
1471 393.1902 392.1829
1472 393.1903 392.1830
1473 393.1904 392.1831
1474 393.1906 392.1833
1475 393.1906 392.1833
1476 393.1909 392.1836
1477 393.1911 392.1838
1478 393.2098 392.2025
1479 393.2981 392.2908
1480 393.2982 392.2909
1481 393.2983 392.2910
1482 393.2983 392.2910
1483 393.2983 392.2910
1484 393.2984 392.2911
1485 393.2988 392.2915
1486 393.2990 392.2917
1487 393.2991 392.2918
1488 393.2991 392.2918
1489 393.2992 392.2919
1490 393.2992 392.2919
1491 393.2993 392.2920
1492 393.2993 392.2920
1493 393.2994 392.2921
1494 393.2994 392.2921
1495 393.2995 392.2922
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