MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1296 377.1599 376.1526
1297 377.1799 376.1726
1298 377.1954 376.1881
1299 377.1955 376.1882
1300 377.1955 376.1882
1301 377.1955 376.1882
1302 377.1959 376.1886
1303 377.1960 376.1887
1304 377.1961 376.1888
1305 377.1961 376.1888
1306 377.2156 376.2083
1307 379.0018 377.9945
1308 379.1369 378.1296
1309 379.1374 378.1301
1310 379.1380 378.1307
1311 379.1380 378.1307
1312 379.1381 378.1308
1313 379.1388 378.1315
1314 379.1391 378.1318
1315 379.1440 378.1367
1316 379.1729 378.1656
1317 379.1731 378.1658
1318 379.1737 378.1664
1319 379.1742 378.1669
1320 379.1743 378.1670
1321 379.1743 378.1670
1322 379.1744 378.1671
1323 379.1744 378.1671
1324 379.1745 378.1672
1325 379.1745 378.1672
1326 379.1746 378.1673
1327 379.1747 378.1674
1328 379.1747 378.1674
1329 379.1748 378.1675
1330 379.1748 378.1675
1331 379.1752 378.1679
1332 379.1753 378.1680
1333 379.1753 378.1680
1334 379.2112 378.2039
1335 379.2112 378.2039
1336 379.2119 378.2046
1337 381.0268 380.0195
1338 381.1523 380.1450
1339 381.1532 380.1459
1340 381.1532 380.1459
1341 381.1533 380.1460
1342 381.1535 380.1462
1343 381.1537 380.1464
1344 381.1537 380.1464
1345 381.1538 380.1465
1346 381.1541 380.1468
1347 381.1542 380.1469
1348 381.1547 380.1474
1349 381.1547 380.1474
1350 381.1891 380.1818
1351 381.1891 380.1818
1352 381.1891 380.1818
1353 381.1892 380.1819
1354 381.1892 380.1819
1355 381.1893 380.1820
1356 381.1893 380.1820
1357 381.1894 380.1821
1358 381.1897 380.1824
1359 381.1898 380.1825
1360 381.1901 380.1828
1361 381.1901 380.1828
1362 381.1901 380.1828
1363 381.1902 380.1829
1364 381.1902 380.1829
1365 381.1904 380.1831
1366 381.1908 380.1835
1367 381.1909 380.1836
1368 381.1909 380.1836
1369 381.1910 380.1837
1370 381.1911 380.1838
1371 381.1913 380.1840
1372 383.0339 382.0266
1373 383.1032 382.0959
1374 383.1335 382.1262
1375 383.1336 382.1263
1376 383.1378 382.1305
1377 383.1682 382.1609
1378 383.1684 382.1611
1379 383.1685 382.1612
1380 383.1685 382.1612
1381 383.1688 382.1615
1382 383.1689 382.1616
1383 383.1692 382.1619
1384 383.1693 382.1620
1385 383.1693 382.1620
1386 383.1694 382.1621
1387 383.1695 382.1622
1388 383.1697 382.1624
1389 383.1700 382.1627
1390 383.1705 382.1632
1391 383.2046 382.1973
1392 383.2051 382.1978
1393 383.2060 382.1987
1394 383.2062 382.1989
1395 383.2063 382.1990
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