MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1096 363.1799 362.1726
1097 363.1799 362.1726
1098 363.1800 362.1727
1099 363.1800 362.1727
1100 363.1801 362.1728
1101 363.1801 362.1728
1102 363.1801 362.1728
1103 363.1801 362.1728
1104 363.1801 362.1728
1105 363.1803 362.1730
1106 363.2006 362.1933
1107 364.2341 363.2268
1108 364.2348 363.2275
1109 364.2348 363.2275
1110 365.0323 364.0250
1111 365.0518 364.0445
1112 365.1214 364.1141
1113 365.1219 364.1146
1114 365.1224 364.1151
1115 365.1577 364.1504
1116 365.1578 364.1505
1117 365.1579 364.1506
1118 365.1579 364.1506
1119 365.1581 364.1508
1120 365.1581 364.1508
1121 365.1582 364.1509
1122 365.1586 364.1513
1123 365.1586 364.1513
1124 365.1586 364.1513
1125 365.1590 364.1517
1126 365.1591 364.1518
1127 365.1592 364.1519
1128 365.1595 364.1522
1129 365.1600 364.1527
1130 365.1943 364.1870
1131 365.1943 364.1870
1132 365.1944 364.1871
1133 365.1945 364.1872
1134 365.1946 364.1873
1135 365.1947 364.1874
1136 365.1949 364.1876
1137 365.1949 364.1876
1138 365.1949 364.1876
1139 365.1952 364.1879
1140 365.1953 364.1880
1141 365.1954 364.1881
1142 365.1955 364.1882
1143 365.1958 364.1885
1144 365.1958 364.1885
1145 365.1958 364.1885
1146 365.1958 364.1885
1147 365.1959 364.1886
1148 365.1960 364.1887
1149 365.1960 364.1887
1150 365.2150 364.2077
1151 367.0470 366.0397
1152 367.0719 366.0646
1153 367.1372 366.1299
1154 367.1373 366.1300
1155 367.1374 366.1301
1156 367.1375 366.1302
1157 367.1376 366.1303
1158 367.1379 366.1306
1159 367.1381 366.1308
1160 367.1382 366.1309
1161 367.1382 366.1309
1162 367.1383 366.1310
1163 367.1733 366.1660
1164 367.1735 366.1662
1165 367.1738 366.1665
1166 367.1739 366.1666
1167 367.1739 366.1666
1168 367.1739 366.1666
1169 367.1740 366.1667
1170 367.1741 366.1668
1171 367.1741 366.1668
1172 367.1742 366.1669
1173 367.1743 366.1670
1174 367.1743 366.1670
1175 367.1747 366.1674
1176 367.1748 366.1675
1177 367.1748 366.1675
1178 367.1749 366.1676
1179 367.1751 366.1678
1180 367.1752 366.1679
1181 367.2077 366.2004
1182 367.2110 366.2037
1183 367.2115 366.2042
1184 367.2115 366.2042
1185 367.2115 366.2042
1186 367.2318 366.2245
1187 367.2318 366.2245
1188 367.2319 366.2246
1189 367.2320 366.2247
1190 367.2321 366.2248
1191 367.2345 366.2272
1192 368.2303 367.2230
1193 368.9651 367.9578
1194 369.0871 368.0798
1195 369.0876 368.0803
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