MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
896 349.2005 348.1932
897 349.2006 348.1933
898 349.2007 348.1934
899 349.2007 348.1934
900 349.2007 348.1934
901 349.2007 348.1934
902 349.2008 348.1935
903 349.2010 348.1937
904 349.2206 348.2133
905 349.2209 348.2136
906 349.2209 348.2136
907 349.2217 348.2144
908 351.0766 350.0693
909 351.1064 350.0991
910 351.1415 350.1342
911 351.1418 350.1345
912 351.1419 350.1346
913 351.1424 350.1351
914 351.1429 350.1356
915 351.1429 350.1356
916 351.1429 350.1356
917 351.1430 350.1357
918 351.1430 350.1357
919 351.1432 350.1359
920 351.1433 350.1360
921 351.1784 350.1711
922 351.1784 350.1711
923 351.1785 350.1712
924 351.1787 350.1714
925 351.1789 350.1716
926 351.1791 350.1718
927 351.1791 350.1718
928 351.1793 350.1720
929 351.1793 350.1720
930 351.1794 350.1721
931 351.1794 350.1721
932 351.1795 350.1722
933 351.1795 350.1722
934 351.1795 350.1722
935 351.1796 350.1723
936 351.1797 350.1724
937 351.1798 350.1725
938 351.1800 350.1727
939 351.1801 350.1728
940 351.1801 350.1728
941 351.1801 350.1728
942 351.1804 350.1731
943 351.1975 350.1902
944 351.1975 350.1902
945 351.1980 350.1907
946 351.2151 350.2078
947 351.2154 350.2081
948 351.2155 350.2082
949 351.2155 350.2082
950 351.2162 350.2089
951 351.2163 350.2090
952 351.2163 350.2090
953 351.2163 350.2090
954 351.2165 350.2092
955 351.2165 350.2092
956 351.2165 350.2092
957 351.2365 350.2292
958 351.2365 350.2292
959 351.2365 350.2292
960 351.2366 350.2293
961 351.2366 350.2293
962 351.2367 350.2294
963 351.9619 350.9546
964 353.0925 352.0852
965 353.1093 352.1020
966 353.1210 352.1137
967 353.1211 352.1138
968 353.1213 352.1140
969 353.1214 352.1141
970 353.1220 352.1147
971 353.1220 352.1147
972 353.1222 352.1149
973 353.1233 352.1160
974 353.1574 352.1501
975 353.1574 352.1501
976 353.1577 352.1504
977 353.1583 352.1510
978 353.1586 352.1513
979 353.1586 352.1513
980 353.1587 352.1514
981 353.1587 352.1514
982 353.1587 352.1514
983 353.1590 352.1517
984 353.1590 352.1517
985 353.1590 352.1517
986 353.1592 352.1519
987 353.1592 352.1519
988 353.1596 352.1523
989 353.1955 352.1882
990 353.9999 352.9926
991 355.0114 354.0041
992 355.0711 354.0638
993 355.0716 354.0643
994 355.1067 354.0994
995 355.1074 354.1001
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