MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
696 337.1274 336.1201
697 337.1276 336.1203
698 337.1277 336.1204
699 337.1284 336.1211
700 337.1626 336.1553
701 337.1626 336.1553
702 337.1628 336.1555
703 337.1628 336.1555
704 337.1633 336.1560
705 337.1634 336.1561
706 337.1634 336.1561
707 337.1635 336.1562
708 337.1635 336.1562
709 337.1636 336.1563
710 337.1636 336.1563
711 337.1636 336.1563
712 337.1637 336.1564
713 337.1637 336.1564
714 337.1639 336.1566
715 337.1640 336.1567
716 337.1642 336.1569
717 337.1645 336.1572
718 337.1817 336.1744
719 337.1824 336.1751
720 337.1825 336.1752
721 337.1826 336.1753
722 337.1829 336.1756
723 338.1606 337.1533
724 338.1830 337.1757
725 338.1834 337.1761
726 339.0756 338.0683
727 339.0762 338.0689
728 339.0769 338.0696
729 339.1045 338.0972
730 339.1053 338.0980
731 339.1055 338.0982
732 339.1060 338.0987
733 339.1081 338.1008
734 339.1131 338.1058
735 339.1133 338.1060
736 339.1216 338.1143
737 339.1217 338.1144
738 339.1301 338.1228
739 339.1416 338.1343
740 339.1418 338.1345
741 339.1418 338.1345
742 339.1420 338.1347
743 339.1422 338.1349
744 339.1423 338.1350
745 339.1424 338.1351
746 339.1424 338.1351
747 339.1427 338.1354
748 339.1428 338.1355
749 339.1429 338.1356
750 339.1429 338.1356
751 339.1429 338.1356
752 339.1430 338.1357
753 339.1431 338.1358
754 339.1432 338.1359
755 339.1435 338.1362
756 339.1435 338.1362
757 339.1786 338.1713
758 339.1788 338.1715
759 339.1790 338.1717
760 339.1792 338.1719
761 339.1793 338.1720
762 339.1799 338.1726
763 339.1985 338.1912
764 339.1985 338.1912
765 341.0912 340.0839
766 341.0915 340.0842
767 341.1096 340.1023
768 341.1099 340.1026
769 341.1212 340.1139
770 341.1214 340.1141
771 341.1215 340.1142
772 341.1216 340.1143
773 341.1216 340.1143
774 341.1224 340.1151
775 341.1233 340.1160
776 341.1576 340.1503
777 341.1576 340.1503
778 341.1577 340.1504
779 341.1578 340.1505
780 341.1579 340.1506
781 341.1580 340.1507
782 341.1580 340.1507
783 341.1580 340.1507
784 341.1580 340.1507
785 341.1586 340.1513
786 341.1587 340.1514
787 341.1588 340.1515
788 341.1588 340.1515
789 341.1594 340.1521
790 341.1954 340.1881
791 341.1955 340.1882
792 341.1956 340.1883
793 341.1958 340.1885
794 341.1960 340.1887
795 341.1966 340.1893
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