MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
596 333.1691 332.1618
597 333.1692 332.1619
598 333.1693 332.1620
599 333.1693 332.1620
600 333.1696 332.1623
601 333.1697 332.1624
602 333.1698 332.1625
603 333.1702 332.1629
604 333.1896 332.1823
605 333.2042 332.1969
606 333.2046 332.1973
607 333.2047 332.1974
608 333.2051 332.1978
609 333.2051 332.1978
610 333.2053 332.1980
611 333.2053 332.1980
612 333.2054 332.1981
613 333.2055 332.1982
614 333.2055 332.1982
615 333.2056 332.1983
616 333.2056 332.1983
617 333.2059 332.1986
618 334.1345 333.1272
619 334.1372 333.1299
620 334.2234 333.2161
621 335.0357 334.0284
622 335.0911 334.0838
623 335.1092 334.1019
624 335.1100 334.1027
625 335.1101 334.1028
626 335.1104 334.1031
627 335.1108 334.1035
628 335.1111 334.1038
629 335.1113 334.1040
630 335.1114 334.1041
631 335.1187 334.1114
632 335.1469 334.1396
633 335.1469 334.1396
634 335.1472 334.1399
635 335.1476 334.1403
636 335.1477 334.1404
637 335.1478 334.1405
638 335.1479 334.1406
639 335.1479 334.1406
640 335.1479 334.1406
641 335.1480 334.1407
642 335.1480 334.1407
643 335.1481 334.1408
644 335.1482 334.1409
645 335.1483 334.1410
646 335.1486 334.1413
647 335.1487 334.1414
648 335.1835 334.1762
649 335.1840 334.1767
650 335.1842 334.1769
651 335.1845 334.1772
652 335.1846 334.1773
653 335.1846 334.1773
654 335.1846 334.1773
655 335.1847 334.1774
656 335.1848 334.1775
657 335.1849 334.1776
658 335.1850 334.1777
659 335.1851 334.1778
660 335.1852 334.1779
661 335.1852 334.1779
662 335.1852 334.1779
663 335.2196 334.2123
664 335.2204 334.2131
665 335.2205 334.2132
666 335.2208 334.2135
667 335.2209 334.2136
668 335.2210 334.2137
669 335.2211 334.2138
670 335.2212 334.2139
671 335.2213 334.2140
672 335.2213 334.2140
673 335.2213 334.2140
674 335.2214 334.2141
675 335.2216 334.2143
676 335.9290 334.9217
677 335.9291 334.9218
678 335.9291 334.9218
679 335.9291 334.9218
680 335.9292 334.9219
681 335.9898 334.9825
682 336.1667 335.1594
683 337.0341 336.0268
684 337.0609 336.0536
685 337.0979 336.0906
686 337.1056 336.0983
687 337.1262 336.1189
688 337.1262 336.1189
689 337.1264 336.1191
690 337.1265 336.1192
691 337.1266 336.1193
692 337.1266 336.1193
693 337.1270 336.1197
694 337.1271 336.1198
695 337.1271 336.1198
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