MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Quantitation overview (42 proteins)

Score Mass Matches Sequences emPAI
Score Mass Matches Sequences emPAI
1.1 Q9EX54_STRCO 4547 46170 190 (159) 41 (36) 76.41 Putative type I polyketide synthase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO6273 PE=4 SV=1
1.2 NARQ_ECOLI 33 0 4 (3) 1 (1) 0.11 description
1.3 A4U8R3_9BACT 23 362145 5 (3) 4 (2) 0.02 SupA OS=Aplysina aerophoba bacterial symbiont clone pAPKS18 GN=supA PE=4 SV=1
2 YHBW_ECOLI 340 37163 15 (13) 10 (9) 1.78 Uncharacterized protein YhbW OS=Escherichia coli (strain K12) GN=yhbW PE=4 SV=1
3 CH60_ECOLI 258 57464 20 (16) 20 (16) 1.44 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2
4 TRYP_PIG 176 25078 6 (5) 4 (4) 0.65 Trypsin OS=Sus scrofa PE=1 SV=1
5 CRP_ECOLI 142 23796 6 (4) 6 (4) 0.69 cAMP-activated global transcriptional regulator CRP OS=Escherichia coli (strain K12) GN=crp PE=1 SV=1
6 K2C1_HUMAN 119 66170 9 (4) 9 (4) 0.21 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6
7 EFTU1_ECOLI 103 43427 3 (3) 1 (1) 0.16 Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1
8 RL4_ECOLI 74 22073 1 (1) 1 (1) 0.15 50S ribosomal protein L4 OS=Escherichia coli (strain K12) GN=rplD PE=1 SV=1
9 ATDA_ECOLI 63 21987 3 (2) 3 (2) 0.33 Spermidine N(1)-acetyltransferase OS=Escherichia coli (strain K12) GN=speG PE=1 SV=2
10 ISPA_ECOLI 61 32368 5 (3) 1 (1) 0.10 Farnesyl diphosphate synthase OS=Escherichia coli (strain K12) GN=ispA PE=1 SV=1
11 RNE_ECOLI 44 118353 6 (3) 6 (3) 0.09 Ribonuclease E OS=Escherichia coli (strain K12) GN=rne PE=1 SV=6
12 LPXA_ECOLI 42 28348 4 (2) 4 (2) 0.25 Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase OS=Escherichia coli (strain K12) GN=lpxA PE=1 SV=1
13 K1C10_HUMAN 33 59020 5 (2) 5 (2) 0.11 Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6
14 GLPB_ECOLI 31 45899 2 (1) 1 (1) 0.07 Anaerobic glycerol-3-phosphate dehydrogenase subunit B OS=Escherichia coli (strain K12) GN=glpB PE=1 SV=1
15 RL7_ECOLI 31 12288 1 (1) 1 (1) 0.28 50S ribosomal protein L7/L12 OS=Escherichia coli (strain K12) GN=rplL PE=1 SV=2
16 OPPF_ECOLI 30 0 3 (2) 2 (1) 0.09 description
17 YNJD_ECOLI 29 24173 3 (2) 1 (1) 0.30 Uncharacterized ABC transporter ATP-binding protein YnjD OS=Escherichia coli (strain K12) GN=ynjD PE=3 SV=2
18 RECB_ECOLI 28 134445 2 (1) 2 (1) 0.02 RecBCD enzyme subunit RecB OS=Escherichia coli (strain K12) GN=recB PE=1 SV=1
19 YBJK_ECOLI 26 0 2 (1) 2 (1) 0.17 description
20 RTCR_ECOLI 26 60604 1 (1) 1 (1) 0.05 Transcriptional regulatory protein RtcR OS=Escherichia coli (strain K12) GN=rtcR PE=4 SV=2
21 VIOA_CHRVO 25 47118 9 (3) 2 (1) 0.15 Probable L-tryptophan oxidase VioA OS=Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) GN=vioA PE=1 SV=2
22 Q92W49_RHIME 24 131031 3 (1) 2 (1) 0.02 Putative methyltransferase OS=Rhizobium meliloti (strain 1021) GN=SM_b20515 PE=4 SV=1
23 YLIE_ECOLI 23 90676 1 (1) 1 (1) 0.04 Putative cyclic di-GMP phosphodiesterase YliE OS=Escherichia coli (strain K12) GN=yliE PE=1 SV=1
24 K1C9_HUMAN 23 62255 7 (2) 2 (2) 0.11 Keratin, type I cytoskeletal 9 OS=Homo sapiens GN=KRT9 PE=1 SV=3
25 GAUTC_ARATH 23 61184 4 (1) 1 (1) 0.05 Probable galacturonosyltransferase 12 OS=Arabidopsis thaliana GN=GAUT12 PE=2 SV=1
26 RPOC_ECOLI 21 155918 2 (1) 2 (1) 0.02 DNA-directed RNA polymerase subunit beta' OS=Escherichia coli (strain K12) GN=rpoC PE=1 SV=1
27 INTF_ECOLI 21 53212 1 (1) 1 (1) 0.06 Putative prophage CP4-6 integrase OS=Escherichia coli (strain K12) GN=intF PE=3 SV=1
28 TREF_ECOLI 21 0 1 (1) 1 (1) 0.05 description
29 FRLR_ECOLI 20 28088 2 (1) 1 (1) 0.12 HTH-type transcriptional regulator FrlR OS=Escherichia coli (strain K12) GN=frlR PE=4 SV=2
30 GLPC_ECOLI 20 45106 1 (1) 1 (1) 0.07 Anaerobic glycerol-3-phosphate dehydrogenase subunit C OS=Escherichia coli (strain K12) GN=glpC PE=1 SV=1
31 LON_ECOLI 20 87725 1 (1) 1 (1) 0.04 Lon protease OS=Escherichia coli (strain K12) GN=lon PE=1 SV=1
32 RFAZ_ECOLI 18 0 1 (1) 1 (1) 0.11 description
33 RSMA_ECOLI 18 30515 4 (1) 1 (1) 0.11 Ribosomal RNA small subunit methyltransferase A OS=Escherichia coli (strain K12) GN=rsmA PE=1 SV=1
34 TTDT_ECOLI 17 53042 3 (1) 2 (1) 0.06 L-tartrate/succinate antiporter OS=Escherichia coli (strain K12) GN=ttdT PE=1 SV=2
35 RS2_ECOLI 16 26784 1 (1) 1 (1) 0.12 30S ribosomal protein S2 OS=Escherichia coli (strain K12) GN=rpsB PE=1 SV=2
36 TAU_HUMAN 16 79108 1 (1) 1 (1) 0.04 Microtubule-associated protein tau OS=Homo sapiens GN=MAPT PE=1 SV=5
37 PSUT_ECOLI 15 0 1 (1) 1 (1) 0.07 description
38 I1MMG1_SOYBN 15 56191 3 (1) 3 (1) 0.06 D13 Uncharacterized protein OS=Glycine max PE=4 SV=1
39 NFRA_ECOLI 14 111468 6 (1) 2 (1) 0.03 Bacteriophage N4 adsorption protein A OS=Escherichia coli (strain K12) GN=nfrA PE=4 SV=1
40 HLYE_ECOLI 13 33852 3 (1) 1 (1) 0.10 Hemolysin E, chromosomal OS=Escherichia coli (strain K12) GN=hlyE PE=1 SV=4

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