MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 40)

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+1

Accession Score Description
Family member distances as a dendrogram 1 Q9EX54_STRCO 4547 Putative type I polyketide synthase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO6273 PE=4 SV=1
2 NARQ_ECOLI 33 description
3 A4U8R3_9BACT 23 SupA OS=Aplysina aerophoba bacterial symbiont clone pAPKS18 GN=supA PE=4 SV=1

+2

Accession Score Description
1 YHBW_ECOLI 340 Uncharacterized protein YhbW OS=Escherichia coli (strain K12) GN=yhbW PE=4 SV=1

+3

Accession Score Description
1 CH60_ECOLI 258 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2

+4

Accession Score Description
1 TRYP_PIG 176 Trypsin OS=Sus scrofa PE=1 SV=1

+5

Accession Score Description
1 CRP_ECOLI 142 cAMP-activated global transcriptional regulator CRP OS=Escherichia coli (strain K12) GN=crp PE=1 SV=1

+6

Accession Score Description
1 K2C1_HUMAN 119 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6

+7

Accession Score Description
1 EFTU1_ECOLI 103 Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1

+8

Accession Score Description
1 RL4_ECOLI 74 50S ribosomal protein L4 OS=Escherichia coli (strain K12) GN=rplD PE=1 SV=1

-9

Accession Score Description
1 ATDA_ECOLI 63 Spermidine N(1)-acetyltransferase OS=Escherichia coli (strain K12) GN=speG PE=1 SV=2
Score Mass Matches Sequences emPAI
9.1 ATDA_ECOLI 63 21987 3 (2) 3 (2) 0.33
Spermidine N(1)-acetyltransferase OS=Escherichia coli (strain K12) GN=speG PE=1 SV=2

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2246   386.7289 771.4432 771.4391 5.33 0 25 0.014 +1Score > 19 indicates identity U K.AIHIYR.K
2852   627.8593 1253.7040 1253.6980 4.86 0 55 6.9e-006 +1Score > 16 indicates identity U K.TPGQTLLKPTAQ.-
D:\Xcalibur\Data\Jennifer\20250522 PRT1249 Qingyun\PRT1249__2.raw

Score > 18 indicates identity
3443   832.4268 1662.8390 1662.8327 3.81 0 10 0.37 -1Score > 18 indicates identity U K.LAMDYGFTVLNLYK.L + Oxidation (M)
-17.9 1 5 1.3 2 KPNPPIKNEADNGLR   + Deamidated (NQ)
4.21 0 3 2 3 VNPGSIILSAEMMLR   + Deamidated (NQ); 2 Oxidation (M)
-13.2 1 3 2 4 SVMLQSLNNIRTLR   + 3 Deamidated (NQ); Oxidation (M)
6.24 0 3 2 5 DSLMLQLYPDAELR  
7.46 1 3 2 5 RLAWQNGHYFTLR   + 2 Deamidated (NQ)
-4.44 0 3 2.1 7 LQENSLFDIVNTIR   + 2 Deamidated (NQ)
-4.44 0 3 2.1 7 LQENSLFDIVNTIR   + 2 Deamidated (NQ)
-4.44 0 3 2.1 7 LQENSLFDIVNTIR   + 2 Deamidated (NQ)
1.49 1 2 2.5 10 ITTFFHTSPDVKNR   + Deamidated (NQ)

+10

Accession Score Description
1 ISPA_ECOLI 61 Farnesyl diphosphate synthase OS=Escherichia coli (strain K12) GN=ispA PE=1 SV=1
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