MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1249__2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues) |
| Timestamp | : | 23 Jun 2025 at 17:45:54 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,602 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q9EX54_STRCO | 4547 | Putative type I polyketide synthase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO6273 PE=4 SV=1 | |
| 2 | NARQ_ECOLI | 33 | description | |
| 3 | A4U8R3_9BACT | 23 | SupA OS=Aplysina aerophoba bacterial symbiont clone pAPKS18 GN=supA PE=4 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YHBW_ECOLI | 340 | Uncharacterized protein YhbW OS=Escherichia coli (strain K12) GN=yhbW PE=4 SV=1 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_ECOLI | 258 | 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 176 | Trypsin OS=Sus scrofa PE=1 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CRP_ECOLI | 142 | cAMP-activated global transcriptional regulator CRP OS=Escherichia coli (strain K12) GN=crp PE=1 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K2C1_HUMAN | 119 | Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 6.1 | K2C1_HUMAN |
119 | 66170 | 9 (4) | 9 (4) | 0.21 | |
| Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6 | |||||||
9 peptide matches (9 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2127 |
337.1985 | 672.3824 | 672.3806 | 2.70 | 0 | 2 | 0.99 | 1Score > 20 indicates identity Score > 15 indicates homology |
U | K.VDLQAK.L | |
D:\Xcalibur\Data\Jennifer\20250522 PRT1249 Qingyun\PRT1249__2.rawScore > 19 indicates identity |
|||||||||||
| 2320 |
437.7553 | 873.4960 | 873.4920 | 4.66 | 0 | 22 | 0.029 | 1Score > 19 indicates identity |
U | R.SLVNLGGSK.S | |
| 4.66 | 0 | 5 | 1.7 | 2 | SIVDITAR | ||||||
| 2473 |
487.2718 | 972.5290 | 972.5240 | 5.22 | 0 | 36 | 0.0015 | 1Score > 20 indicates identity |
U | K.IEISELNR.V | |
| 2581 |
517.2636 | 1032.5126 | 1032.5087 | 3.80 | 0 | 17 | 0.081 | 1Score > 19 indicates identity |
U | R.TLLEGEESR.M | |
| 2622 |
533.2657 | 1064.5168 | 1064.5138 | 2.85 | 0 | 9 | 0.54 | 1Score > 18 indicates identity |
U | K.AQYEDIAQK.S | |
| 2960 |
651.8638 | 1301.7130 | 1301.7078 | 4.01 | 0 | 72 | 2.3e-007 | 1Score > 18 indicates identity |
U | R.SLDLDSIIAEVK.A | |
| 3127 |
692.3486 | 1382.6826 | 1382.6830 | -0.26 | 0 | 2 | 2.3 | 1Score > 18 indicates identity |
U | K.SLNNQFASFIDK.V | |
| 3152 |
465.2493 | 1392.7261 | 1392.7249 | 0.86 | 1 | 17 | 0.073 | 1Score > 18 indicates identity |
U | R.TNAENEFVTIKK.D | |
| 3302 |
738.3991 | 1474.7836 | 1474.7780 | 3.84 | 0 | 46 | 9.8e-005 | 1Score > 18 indicates identity |
U | R.FLEQQNQVLQTK.W | |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| K22E_HUMAN |
63 | 65678 | 6.1 | ||
| Keratin, type II cytoskeletal 2 epidermal OS=Homo sapiens GN=KRT2 PE=1 SV=2 | |||||
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU1_ECOLI | 103 | Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL4_ECOLI | 74 | 50S ribosomal protein L4 OS=Escherichia coli (strain K12) GN=rplD PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATDA_ECOLI | 63 | Spermidine N(1)-acetyltransferase OS=Escherichia coli (strain K12) GN=speG PE=1 SV=2 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ISPA_ECOLI | 61 | Farnesyl diphosphate synthase OS=Escherichia coli (strain K12) GN=ispA PE=1 SV=1 |
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