MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 40)

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+31

Accession Score Description
1 LON_ECOLI 20 Lon protease OS=Escherichia coli (strain K12) GN=lon PE=1 SV=1

+32

Accession Score Description
1 RFAZ_ECOLI 18 description

+33

Accession Score Description
1 RSMA_ECOLI 18 Ribosomal RNA small subunit methyltransferase A OS=Escherichia coli (strain K12) GN=rsmA PE=1 SV=1

+34

Accession Score Description
1 TTDT_ECOLI 17 L-tartrate/succinate antiporter OS=Escherichia coli (strain K12) GN=ttdT PE=1 SV=2

+35

Accession Score Description
1 RS2_ECOLI 16 30S ribosomal protein S2 OS=Escherichia coli (strain K12) GN=rpsB PE=1 SV=2

+36

Accession Score Description
1 TAU_HUMAN 16 Microtubule-associated protein tau OS=Homo sapiens GN=MAPT PE=1 SV=5

+37

Accession Score Description
1 PSUT_ECOLI 15 description

+38

Accession Score Description
1 I1MMG1_SOYBN 15 D13 Uncharacterized protein OS=Glycine max PE=4 SV=1

+39

Accession Score Description
1 NFRA_ECOLI 14 Bacteriophage N4 adsorption protein A OS=Escherichia coli (strain K12) GN=nfrA PE=4 SV=1

-40

Accession Score Description
1 HLYE_ECOLI 13 Hemolysin E, chromosomal OS=Escherichia coli (strain K12) GN=hlyE PE=1 SV=4
Score Mass Matches Sequences emPAI
40.1 HLYE_ECOLI 13 33852 3 (1) 1 (1) 0.10
Hemolysin E, chromosomal OS=Escherichia coli (strain K12) GN=hlyE PE=1 SV=4

-3 peptide matches (2 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2984 +1 439.8582 1316.5528 1316.5377 11.5 0 13 0.045 +1Score > 13 indicates identity U K.MINTCNEYQK.R + Deamidated (NQ); Oxidation (M)
2985   659.2853 1316.5560 1316.5377 14.0 0 9 0.13 +1Score > 13 indicates identity U K.MINTCNEYQK.R + Deamidated (NQ); Oxidation (M)
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