MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1249__2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues) |
| Timestamp | : | 23 Jun 2025 at 17:45:54 GMT |
Not what you expected? Try 
the select summary.
| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,602 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q9EX54_STRCO | 4547 | Putative type I polyketide synthase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO6273 PE=4 SV=1 | |
| 2 | NARQ_ECOLI | 33 | description | |
| 3 | A4U8R3_9BACT | 23 | SupA OS=Aplysina aerophoba bacterial symbiont clone pAPKS18 GN=supA PE=4 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YHBW_ECOLI | 340 | Uncharacterized protein YhbW OS=Escherichia coli (strain K12) GN=yhbW PE=4 SV=1 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_ECOLI | 258 | 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 176 | Trypsin OS=Sus scrofa PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 4.1 | TRYP_PIG |
176 | 25078 | 6 (5) | 4 (4) | 0.65 | |
| Trypsin OS=Sus scrofa PE=1 SV=1 | |||||||
6 peptide matches (5 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2301 |
1 |
421.7604 | 841.5062 | 841.5022 | 4.87 | 0 | 53 | 9.8e-006 | 1Score > 16 indicates identity |
U | R.VATVSLPR.S |
| 2595 |
523.2878 | 1044.5610 | 1044.5564 | 4.50 | 0 | 56 | 1.5e-005 | 1Score > 20 indicates identity |
U | K.LSSPATLNSR.V | |
| 4131 |
738.0430 | 2211.1072 | 2211.0807 | 12.0 | 0 | 64 | 1.5e-006 | 1Score > 19 indicates identity |
U | R.LGEHNIDVLEGNEQFINAAK.I + Deamidated (NQ) | |
| 4153 |
767.4008 | 2299.1806 | 2299.1518 | 12.5 | 0 | 53 | 2.2e-005 | 1Score > 18 indicates identity |
U | K.IITHPNFNGNTLDNDIMLIK.L + Deamidated (NQ); Oxidation (M) | |
D:\Xcalibur\Data\Jennifer\20250522 PRT1249 Qingyun\PRT1249__2.rawScore > 19 indicates identity |
|||||||||||
| 4154 |
767.7284 | 2300.1634 | 2300.1358 | 12.0 | 0 | 10 | 0.46 | 1Score > 19 indicates identity |
U | K.IITHPNFNGNTLDNDIMLIK.L + 2 Deamidated (NQ); Oxidation (M) | |
| 12.0 | 0 | 5 | 1.5 | 2 | K.IITHPNFNGNTLDNDIMLIK.L + 2 Deamidated (NQ); Oxidation (M) | ||||||
| 12.0 | 0 | 4 | 1.7 | 3 | K.IITHPNFNGNTLDNDIMLIK.L + 2 Deamidated (NQ); Oxidation (M) | ||||||
| 12.0 | 0 | 2 | 2.8 | 4 | K.IITHPNFNGNTLDNDIMLIK.L + 2 Deamidated (NQ); Oxidation (M) | ||||||
| 12.0 | 0 | 1 | 3.5 | 5 | K.IITHPNFNGNTLDNDIMLIK.L + 2 Deamidated (NQ); Oxidation (M) | ||||||
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CRP_ECOLI | 142 | cAMP-activated global transcriptional regulator CRP OS=Escherichia coli (strain K12) GN=crp PE=1 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K2C1_HUMAN | 119 | Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU1_ECOLI | 103 | Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL4_ECOLI | 74 | 50S ribosomal protein L4 OS=Escherichia coli (strain K12) GN=rplD PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATDA_ECOLI | 63 | Spermidine N(1)-acetyltransferase OS=Escherichia coli (strain K12) GN=speG PE=1 SV=2 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ISPA_ECOLI | 61 | Farnesyl diphosphate synthase OS=Escherichia coli (strain K12) GN=ispA PE=1 SV=1 |
Not what you expected? Try the select summary.
Mascot: http://www.matrixscience.com/