MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1249__2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues) |
| Timestamp | : | 23 Jun 2025 at 17:45:54 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,602 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RNE_ECOLI | 44 | Ribonuclease E OS=Escherichia coli (strain K12) GN=rne PE=1 SV=6 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 11.1 | RNE_ECOLI |
44 | 118353 | 6 (3) | 6 (3) | 0.09 | |
| Ribonuclease E OS=Escherichia coli (strain K12) GN=rne PE=1 SV=6 | |||||||
6 peptide matches (6 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2168 |
350.7223 | 699.4300 | 699.4279 | 3.07 | 0 | 14 | 0.1 | 1Score > 16 indicates identity |
U | K.VLELAR.Q | |
| 2222 |
372.2249 | 742.4352 | 742.4449 | -13.1 | 1 | 4 | 1.8 | 7Score > 19 indicates identity |
U | R.LREAVR.Q | |
| 2265 |
400.7089 | 799.4032 | 799.4188 | -19.4 | 0 | 4 | 0.55 | 1Score > 13 indicates identity |
U | K.AEAKPER.Q | |
| 2309 |
429.2478 | 856.4810 | 856.4767 | 5.11 | 0 | 44 | 0.00014 | 1Score > 18 indicates identity |
U | R.VALVDGQR.L | |
| 2441 |
318.5239 | 952.5499 | 952.5454 | 4.70 | 0 | 14 | 0.038 | 1Score > 13 indicates identity |
U | R.IQISHISR.F | |
| 3454 |
557.9590 | 1670.8552 | 1670.8475 | 4.59 | 1 | 18 | 0.055 | 1Score > 18 indicates identity |
U | R.EGQEVIVQIDKEER.G |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LPXA_ECOLI | 42 | Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase OS=Escherichia coli (strain K12) GN=lpxA PE=1 SV=1 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K1C10_HUMAN | 33 | Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLPB_ECOLI | 31 | Anaerobic glycerol-3-phosphate dehydrogenase subunit B OS=Escherichia coli (strain K12) GN=glpB PE=1 SV=1 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL7_ECOLI | 31 | 50S ribosomal protein L7/L12 OS=Escherichia coli (strain K12) GN=rplL PE=1 SV=2 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OPPF_ECOLI | 30 | description |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YNJD_ECOLI | 29 | Uncharacterized ABC transporter ATP-binding protein YnjD OS=Escherichia coli (strain K12) GN=ynjD PE=3 SV=2 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RECB_ECOLI | 28 | RecBCD enzyme subunit RecB OS=Escherichia coli (strain K12) GN=recB PE=1 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YBJK_ECOLI | 26 | description |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RTCR_ECOLI | 26 | Transcriptional regulatory protein RtcR OS=Escherichia coli (strain K12) GN=rtcR PE=4 SV=2 |
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