MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
356 317.0916 316.0843
357 317.0988 316.0915
358 317.0998 316.0925
359 317.1000 316.0927
360 317.1001 316.0928
361 317.1003 316.0930
362 317.1007 316.0934
363 317.1015 316.0942
364 317.1291 316.1218
365 317.1367 316.1294
366 317.1372 316.1299
367 317.1378 316.1305
368 317.1378 316.1305
369 317.1743 316.1670
370 317.1745 316.1672
371 317.1745 316.1672
372 317.1747 316.1674
373 317.1748 316.1675
374 317.1764 316.1691
375 317.1943 316.1870
376 317.1948 316.1875
377 317.1954 316.1881
378 318.0428 317.0355
379 318.1922 317.1849
380 318.1922 317.1849
381 318.1926 317.1853
382 318.1927 317.1854
383 318.8783 317.8710
384 318.8785 317.8712
385 319.0406 318.0333
386 319.0415 318.0342
387 319.0495 318.0422
388 319.0800 318.0727
389 319.1152 318.1079
390 319.1157 318.1084
391 319.1161 318.1088
392 319.1161 318.1088
393 319.1163 318.1090
394 319.1163 318.1090
395 319.1165 318.1092
396 319.1169 318.1096
397 319.1169 318.1096
398 319.1171 318.1098
399 319.1524 318.1451
400 319.1529 318.1456
401 319.1529 318.1456
402 319.1531 318.1458
403 319.1531 318.1458
404 319.1534 318.1461
405 319.1534 318.1461
406 319.1535 318.1462
407 319.1535 318.1462
408 319.1535 318.1462
409 319.1536 318.1463
410 319.1536 318.1463
411 319.1537 318.1464
412 319.1537 318.1464
413 319.1538 318.1465
414 319.1538 318.1465
415 319.1893 318.1820
416 319.1898 318.1825
417 319.1901 318.1828
418 319.1901 318.1828
419 319.1902 318.1829
420 319.1903 318.1830
421 319.1903 318.1830
422 319.1906 318.1833
423 319.2264 318.2191
424 319.2266 318.2193
425 319.2267 318.2194
426 319.2270 318.2197
427 319.9946 318.9873
428 319.9953 318.9880
429 321.0649 320.0576
430 321.0672 320.0599
431 321.0934 320.0861
432 321.0938 320.0865
433 321.0941 320.0868
434 321.0944 320.0871
435 321.0945 320.0872
436 321.0950 320.0877
437 321.0952 320.0879
438 321.0956 320.0883
439 321.0960 320.0887
440 321.1301 320.1228
441 321.1306 320.1233
442 321.1310 320.1237
443 321.1316 320.1243
444 321.1317 320.1244
445 321.1317 320.1244
446 321.1318 320.1245
447 321.1318 320.1245
448 321.1319 320.1246
449 321.1320 320.1247
450 321.1320 320.1247
451 321.1320 320.1247
452 321.1320 320.1247
453 321.1321 320.1248
454 321.1327 320.1254
455 321.1328 320.1255
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