MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
256 311.0831 310.0758
257 311.0900 310.0827
258 311.0907 310.0834
259 311.0908 310.0835
260 311.0999 310.0926
261 311.1103 310.1030
262 311.1109 310.1036
263 311.1109 310.1036
264 311.1110 310.1037
265 311.1113 310.1040
266 311.1113 310.1040
267 311.1115 310.1042
268 311.1116 310.1043
269 311.1116 310.1043
270 311.1117 310.1044
271 311.1473 310.1400
272 311.1475 310.1402
273 311.1477 310.1404
274 311.1477 310.1404
275 311.1479 310.1406
276 311.1479 310.1406
277 311.1479 310.1406
278 311.1481 310.1408
279 311.1481 310.1408
280 311.1482 310.1409
281 311.1483 310.1410
282 311.1484 310.1411
283 311.1484 310.1411
284 311.1485 310.1412
285 311.1485 310.1412
286 311.1485 310.1412
287 311.1659 310.1586
288 311.1662 310.1589
289 311.1664 310.1591
290 311.1665 310.1592
291 311.1842 310.1769
292 311.1843 310.1770
293 311.1850 310.1777
294 311.2233 310.2160
295 311.2235 310.2162
296 312.1323 311.1250
297 312.1326 311.1253
298 313.0217 312.0144
299 313.0601 312.0528
300 313.0608 312.0535
301 313.0608 312.0535
302 313.0609 312.0536
303 313.0905 312.0832
304 313.0967 312.0894
305 313.0971 312.0898
306 313.0979 312.0906
307 313.1271 312.1198
308 313.1275 312.1202
309 313.1276 312.1203
310 313.1284 312.1211
311 313.1288 312.1215
312 313.1291 312.1218
313 313.1632 312.1559
314 313.1635 312.1562
315 313.1635 312.1562
316 313.1638 312.1565
317 313.1640 312.1567
318 313.1642 312.1569
319 313.1645 312.1572
320 313.1646 312.1573
321 313.1647 312.1574
322 313.1653 312.1580
323 313.1828 312.1755
324 313.1834 312.1761
325 313.1835 312.1762
326 313.1835 312.1762
327 314.0466 313.0393
328 314.0467 313.0394
329 314.1311 313.1238
330 314.1973 313.1900
331 314.1976 313.1903
332 314.1982 313.1909
333 315.0399 314.0326
334 315.0753 314.0680
335 315.0756 314.0683
336 315.0756 314.0683
337 315.0758 314.0685
338 315.0765 314.0692
339 315.1135 314.1062
340 315.1145 314.1072
341 315.1413 314.1340
342 315.1425 314.1352
343 315.1428 314.1355
344 315.1431 314.1358
345 315.1434 314.1361
346 315.1586 314.1513
347 315.1601 314.1528
348 315.1790 314.1717
349 315.1793 314.1720
350 316.0255 315.0182
351 316.1753 315.1680
352 316.1990 315.1917
353 316.2130 315.2057
354 316.2139 315.2066
355 317.0548 316.0475
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