MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
156 307.1526 306.1453
157 307.1528 306.1455
158 307.1529 306.1456
159 307.1529 306.1456
160 307.1529 306.1456
161 307.1529 306.1456
162 307.1530 306.1457
163 307.1532 306.1459
164 307.1532 306.1459
165 307.1533 306.1460
166 307.1534 306.1461
167 307.1535 306.1462
168 307.1535 306.1462
169 307.1536 306.1463
170 307.1537 306.1464
171 307.1538 306.1465
172 307.1889 306.1816
173 307.1893 306.1820
174 307.1895 306.1822
175 307.1897 306.1824
176 307.1902 306.1829
177 307.1903 306.1830
178 307.1904 306.1831
179 307.1904 306.1831
180 307.1904 306.1831
181 307.1905 306.1832
182 307.1905 306.1832
183 307.1906 306.1833
184 309.0054 307.9981
185 309.0663 308.0590
186 309.0948 308.0875
187 309.0949 308.0876
188 309.0951 308.0878
189 309.0951 308.0878
190 309.0951 308.0878
191 309.0951 308.0878
192 309.0953 308.0880
193 309.0954 308.0881
194 309.0962 308.0889
195 309.1316 308.1243
196 309.1317 308.1244
197 309.1317 308.1244
198 309.1317 308.1244
199 309.1317 308.1244
200 309.1318 308.1245
201 309.1319 308.1246
202 309.1321 308.1248
203 309.1322 308.1249
204 309.1322 308.1249
205 309.1323 308.1250
206 309.1324 308.1251
207 309.1324 308.1251
208 309.1324 308.1251
209 309.1324 308.1251
210 309.1324 308.1251
211 309.1325 308.1252
212 309.1325 308.1252
213 309.1326 308.1253
214 309.1327 308.1254
215 309.1327 308.1254
216 309.1677 308.1604
217 309.1681 308.1608
218 309.1682 308.1609
219 309.1682 308.1609
220 309.1683 308.1610
221 309.1684 308.1611
222 309.1685 308.1612
223 309.1685 308.1612
224 309.1685 308.1612
225 309.1686 308.1613
226 309.1686 308.1613
227 309.1686 308.1613
228 309.1688 308.1615
229 309.1692 308.1619
230 309.1692 308.1619
231 309.1693 308.1620
232 309.1693 308.1620
233 309.1693 308.1620
234 309.1693 308.1620
235 309.1695 308.1622
236 309.1695 308.1622
237 309.1695 308.1622
238 309.1701 308.1628
239 309.1883 308.1810
240 309.1884 308.1811
241 309.1884 308.1811
242 309.1886 308.1813
243 309.2057 308.1984
244 309.2057 308.1984
245 309.2063 308.1990
246 309.2064 308.1991
247 309.2065 308.1992
248 309.2066 308.1993
249 309.4840 308.4767
250 310.0155 309.0082
251 311.0450 310.0377
252 311.0810 310.0737
253 311.0816 310.0743
254 311.0816 310.0743
255 311.0816 310.0743
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