MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4101–4200 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4389 892.4671 2674.3795
4390 893.4689 2677.3849
4393 898.7783 2693.3131
4395 675.8415 2699.3369
4396 900.7865 2699.3377
4397 902.1098 2703.3076
4399 682.3120 2725.2189
4400 909.7488 2726.2246
4409 920.7953 2759.3641
4411 692.8309 2767.2945
4414 929.1996 2784.5770
4416 935.5117 2803.5133
4417 702.8406 2807.3333
4418 947.4794 2839.4164
4419 950.8156 2849.4250
4420 956.1263 2865.3571
4422 970.1567 2907.4483
4423 971.8465 2912.5177
4424 730.1287 2916.4857
4425 730.1292 2916.4877
4427 979.5032 2935.4878
4428 734.8796 2935.4893
4430 734.8817 2935.4977
4431 734.8842 2935.5077
4433 590.0864 2945.3956
4434 983.1937 2946.5593
4435 983.1964 2946.5674
4436 983.8151 2948.4235
4437 738.8719 2951.4585
4438 592.4994 2957.4606
4439 740.3745 2957.4689
4440 741.8782 2963.4837
4441 741.8796 2963.4893
4442 989.1683 2964.4831
4443 989.1708 2964.4906
4444 744.3660 2973.4349
4445 595.6953 2973.4401
4446 496.5809 2973.4417
4447 595.8953 2974.4401
4448 748.8778 2991.4821
4449 601.2931 3001.4291
4450 1007.5602 3019.6588
4451 1046.5709 3136.6909
4456 1076.5260 3226.5562
4457 1111.5548 3331.6426
4459 1118.8779 3353.6119
4460 843.4041 3369.5873
4462 856.4359 3421.7145
4463 686.1463 3425.6951
4464 857.4313 3425.6961
4465 857.4319 3425.6985
4466 686.5471 3427.6991
4467 886.2482 3540.9637
4471 943.2515 3768.9769
4472 973.7245 3890.8689
4474 986.0004 3939.9725
4475 993.7477 3970.9617
4476 1010.2688 4037.0461
4477 813.4233 4062.0801
4479 695.5406 4167.1999
4480 695.5411 4167.2029
4481 834.4500 4167.2136
4482 1051.0356 4200.1133
4483 1055.0344 4216.1085
4484 1060.7780 4239.0829
4485 1153.8361 4611.3153
4487 1108.5105 5537.5161
4488 1109.9010 5544.4686
4489 1110.1001 5545.4641
4490 1111.7075 5553.5011
4491 1111.7085 5553.5061
4492 1111.9098 5554.5126
4493 927.6580 5559.9043
4494 1113.1018 5560.4726
4495 1113.1042 5560.4846
4496 927.8214 5560.8847
4497 1113.2972 5561.4496
4498 1114.4966 5567.4466
4499 1114.7056 5568.4916
4500 1114.9042 5569.4846
4501 1114.9076 5569.5016
4502 1114.9111 5569.5191
4503 929.4219 5570.4877
4504 1115.1055 5570.4911
4505 1116.1024 5575.4756
4506 1116.1036 5575.4816
4507 930.3219 5575.8877
4508 1116.2974 5576.4506
4509 930.4218 5576.4871
4510 1117.4930 5582.4286
4511 1117.4934 5582.4306
4512 1118.1033 5585.4801
4513 1118.1062 5585.4946
4514 1118.3040 5586.4836
4515 932.7491 5590.4509
4516 932.9157 5591.4505
4517 1119.3000 5591.4636
4518 932.9185 5591.4673
4519 932.9191 5591.4709
4520 1119.5012 5592.4696
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