MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4001–4100 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4260 820.7772 2459.3098
4261 616.2968 2461.1581
4262 493.2410 2461.1686
4263 494.6426 2468.1766
4264 618.5729 2470.2625
4267 497.0491 2480.2091
4268 621.0605 2480.2129
4269 829.4346 2485.2820
4270 498.6438 2488.1826
4271 830.7521 2489.2345
4273 832.0827 2493.2263
4274 832.0850 2493.2332
4275 834.4194 2500.2364
4276 837.0856 2508.2350
4278 839.1003 2514.2791
4279 629.5890 2514.3269
4280 839.4066 2515.1980
4281 629.8345 2515.3089
4283 630.0574 2516.2005
4284 505.0636 2520.2816
4285 505.0645 2520.2861
4286 631.0792 2520.2877
4287 505.0661 2520.2941
4288 631.3272 2521.2797
4289 841.7771 2522.3095
4290 633.8009 2531.1745
4291 633.8011 2531.1753
4294 637.0850 2544.3109
4295 637.5532 2546.1837
4296 510.4448 2547.1876
4297 638.3291 2549.2873
4299 638.8250 2551.2709
4300 511.4618 2552.2726
4301 511.4622 2552.2746
4302 852.4948 2554.4626
4314 854.7832 2561.3278
4315 855.4357 2563.2853
4316 856.4322 2566.2748
4317 642.8276 2567.2813
4318 643.8310 2571.2949
4319 859.4355 2575.2847
4320 644.8290 2575.2869
4321 859.4387 2575.2943
4322 859.4388 2575.2946
4323 644.8311 2575.2953
4324 859.4647 2575.3723
4327 517.2595 2581.2611
4328 646.3229 2581.2625
4330 646.8224 2583.2605
4331 863.4346 2587.2820
4332 863.4352 2587.2838
4333 863.4354 2587.2844
4334 647.8300 2587.2909
4335 863.7574 2588.2504
4336 863.7716 2588.2930
4337 864.7682 2591.2828
4338 864.7692 2591.2858
4339 864.7702 2591.2888
4340 648.8295 2591.2889
4341 648.8298 2591.2901
4342 520.4548 2597.2376
4343 866.7532 2597.2378
4344 650.3171 2597.2393
4345 650.3175 2597.2409
4346 520.4556 2597.2416
4347 520.4602 2597.2646
4348 867.0872 2598.2398
4349 867.0961 2598.2665
4350 650.8231 2599.2633
4351 651.8193 2603.2481
4352 868.7608 2603.2606
4353 868.7687 2603.2843
4354 869.4409 2605.3009
4355 871.4247 2611.2523
4357 654.3137 2613.2257
4358 872.0870 2613.2392
4360 872.4169 2614.2289
4361 523.8565 2614.2461
4363 872.4258 2614.2556
4364 873.1106 2616.3100
4365 874.0746 2619.2020
4366 655.8171 2619.2393
4367 874.1005 2619.2797
4368 874.4050 2620.1932
4369 525.0475 2620.2011
4370 874.7390 2621.1952
4372 875.1093 2622.3061
4373 657.5648 2626.2301
4375 658.3156 2629.2333
4377 879.3996 2635.1770
4378 659.8027 2635.1817
4379 528.0438 2635.1826
4380 879.4749 2635.4029
4381 529.2468 2641.1976
4382 661.8193 2643.2481
4383 663.8041 2651.1873
4385 665.3156 2657.2333
4386 888.1371 2661.3895
4387 889.4487 2665.3243
4388 669.2906 2673.1333
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