MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3901–4000 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4086 403.8019 2013.9731
4087 672.3319 2013.9739
4088 504.5008 2013.9741
4091 672.3562 2014.0468
4096 674.0037 2018.9893
4097 1011.0000 2019.9854
4098 674.3362 2019.9868
4099 506.0050 2019.9909
4100 675.3570 2023.0492
4101 507.2470 2024.9589
4102 676.3383 2025.9931
4103 676.3423 2026.0051
4104 677.3188 2028.9346
4105 508.2418 2028.9381
4111 678.3370 2031.9892
4112 678.3381 2031.9925
4113 678.3578 2032.0516
4114 509.2568 2032.9981
4117 679.6546 2035.9420
4123 511.2499 2040.9705
4124 511.2517 2040.9777
4126 681.6631 2041.9675
4127 681.6641 2041.9705
4132 1024.4967 2046.9788
4133 683.3336 2046.9790
4134 410.4031 2046.9791
4137 1026.5099 2051.0052
4138 684.9803 2051.9191
4139 685.3600 2053.0582
4140 514.5049 2053.9905
4141 1028.0349 2054.0552
4142 685.6927 2054.0563
4143 515.2409 2056.9345
4144 1030.0192 2058.0238
4150 688.3229 2061.9469
4153 1035.4849 2068.9552
4155 691.9988 2072.9746
4156 1037.9941 2073.9736
4159 693.0234 2076.0484
4160 520.4937 2077.9457
4161 694.6891 2081.0455
4162 695.6474 2083.9204
4164 697.0256 2088.0550
4165 523.4918 2089.9381
4166 697.9881 2090.9425
4167 419.2080 2091.0036
4168 523.7587 2091.0057
4169 698.3430 2092.0072
4172 1062.5267 2123.0388
4176 724.7225 2171.1457
4177 1086.5822 2171.1498
4178 546.0205 2180.0529
4180 737.3715 2209.0927
4182 747.7192 2240.1358
4184 1123.0994 2244.1842
4185 563.2703 2249.0521
4186 752.0754 2253.2044
4187 752.3529 2254.0369
4188 568.2722 2269.0597
4193 764.4179 2290.2319
4196 768.0650 2301.1732
4197 578.7904 2311.1325
4199 777.7125 2330.1157
4200 780.0832 2337.2278
4201 782.0940 2343.2602
4202 786.0724 2355.1954
4203 786.4064 2356.1974
4209 1194.1409 2386.2672
4216 600.3188 2397.2461
4218 482.8547 2409.2371
4219 603.3182 2409.2437
4220 604.5723 2414.2601
4221 805.7612 2414.2618
4222 805.7616 2414.2630
4224 604.5746 2414.2693
4228 807.1138 2418.3196
4229 485.6512 2423.2196
4230 809.0775 2424.2107
4231 405.0429 2424.2137
4232 607.0610 2424.2149
4233 485.8503 2424.2151
4235 486.8527 2429.2271
4236 811.0842 2430.2308
4237 487.0540 2430.2336
4238 608.5659 2430.2345
4240 813.0879 2436.2419
4241 813.1318 2436.3736
4243 610.3150 2437.2309
4246 815.0947 2442.2623
4248 611.5735 2442.2649
4249 815.4276 2443.2610
4250 611.8314 2443.2965
4251 491.2480 2451.2036
4252 613.8114 2451.2165
4253 818.4092 2452.2058
4254 614.0591 2452.2073
4255 491.4489 2452.2081
4256 614.0602 2452.2117
4258 615.3125 2457.2209
4259 492.4518 2457.2226
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