MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3940 952.4399 1902.8652
3941 952.4493 1902.8840
3942 953.9387 1905.8628
3944 636.2961 1905.8665
3948 637.9606 1910.8600
3949 956.9174 1911.8202
3950 478.9636 1911.8253
3952 638.6151 1912.8235
3953 638.6153 1912.8241
3954 638.9532 1913.8378
3955 640.2856 1917.8350
3956 959.9404 1917.8662
3957 960.9443 1919.8740
3958 640.9737 1919.8993
3960 481.2327 1920.9017
3961 481.2331 1920.9033
3962 641.6261 1921.8565
3965 482.9619 1927.8185
3966 643.6136 1927.8190
3967 643.9529 1928.8369
3970 967.9390 1933.8634
3971 484.9535 1935.7849
3972 646.2697 1935.7873
3974 969.9337 1937.8528
3975 970.4354 1938.8562
3976 970.4580 1938.9014
3977 485.7327 1938.9017
3978 647.3081 1938.9025
3979 647.3099 1938.9079
3981 485.9608 1939.8141
3983 485.9841 1939.9073
3984 647.9465 1940.8177
3985 649.2795 1944.8167
3986 487.2117 1944.8177
3988 390.5948 1947.9376
3993 488.2095 1948.8089
3994 488.2432 1948.9437
3996 488.4491 1949.7673
3997 975.9336 1949.8526
3998 975.9351 1949.8556
3999 651.2640 1950.7702
4000 976.9403 1951.8660
4001 652.9345 1955.7817
4003 653.6796 1958.0170
4005 490.7202 1958.8517
4006 980.5121 1959.0096
4007 654.0118 1959.0136
4008 654.0125 1959.0157
4009 490.9588 1959.8061
4010 654.6088 1960.8046
4011 655.2950 1962.8632
4014 658.0114 1971.0124
4015 659.3471 1975.0195
4017 659.3510 1975.0312
4018 659.3517 1975.0333
4019 494.9581 1975.8033
4020 659.6804 1976.0194
4022 659.6818 1976.0236
4023 659.6822 1976.0248
4024 989.0199 1976.0252
4025 495.0137 1976.0257
4026 989.0202 1976.0258
4027 659.6829 1976.0269
4028 659.6830 1976.0272
4030 659.6846 1976.0320
4031 659.6848 1976.0326
4033 659.6849 1976.0329
4034 659.6852 1976.0338
4037 659.9427 1976.8063
4038 659.9580 1976.8522
4039 495.2244 1976.8685
4040 396.5786 1977.8566
4041 495.4720 1977.8589
4043 496.5014 1981.9765
4045 663.3381 1986.9925
4046 663.3418 1987.0036
4049 663.3450 1987.0132
4052 499.9901 1995.9313
4053 499.9965 1995.9569
4054 400.3992 1996.9596
4055 666.6607 1996.9603
4056 400.6058 1997.9926
4057 667.0089 1998.0049
4059 667.0115 1998.0127
4061 667.3386 1998.9940
4062 501.5000 2001.9709
4063 668.6808 2003.0206
4064 669.0135 2004.0187
4065 669.0141 2004.0205
4066 1003.0176 2004.0206
4067 1003.0197 2004.0248
4068 669.0165 2004.0277
4069 669.0165 2004.0277
4070 669.3583 2005.0531
4075 503.2491 2008.9673
4079 670.6857 2009.0353
4082 504.2481 2012.9633
4083 671.9958 2012.9656
4084 1007.4919 2012.9692
4085 403.6015 2012.9711
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