MASCOT Search Results

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User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3664 818.4117 1634.8088
3665 819.4561 1636.8976
3666 819.9521 1637.8896
3668 819.9588 1637.9030
3670 549.6221 1645.8445
3671 550.6285 1648.8637
3672 830.4241 1658.8336
3675 835.4083 1668.8020
3676 557.6524 1669.9354
3677 837.4468 1672.8790
3678 559.3079 1674.9019
3680 419.9694 1675.8485
3681 559.9602 1676.8588
3682 559.9604 1676.8594
3685 566.3260 1695.9562
3686 850.3887 1698.7628
3687 850.3979 1698.7812
3702 572.3329 1713.9769
3705 858.9036 1715.7926
3706 859.3964 1716.7782
3707 573.2728 1716.7966
3715 576.6339 1726.8799
3717 577.3286 1728.9640
3718 865.9409 1729.8672
3719 866.8800 1731.7454
3720 867.3998 1732.7850
3728 869.8913 1737.7680
3729 869.9395 1737.8644
3730 870.3870 1738.7594
3731 580.5962 1738.7668
3732 580.9244 1739.7514
3733 871.4911 1740.9676
3734 581.3309 1740.9709
3735 581.3315 1740.9727
3736 581.3324 1740.9754
3737 871.9119 1741.8092
3738 581.6638 1741.9696
3739 871.9932 1741.9718
3740 871.9932 1741.9718
3741 871.9938 1741.9730
3744 581.6672 1741.9798
3745 872.9034 1743.7922
3746 873.3922 1744.7698
3747 874.4940 1746.9734
3748 874.8792 1747.7438
3749 874.9023 1747.7900
3753 584.6468 1750.9186
3754 438.7373 1750.9201
3755 876.9637 1751.9128
3757 351.3907 1751.9171
3758 876.9669 1751.9192
3759 584.9813 1751.9221
3761 584.9817 1751.9233
3763 877.4199 1752.8252
3764 877.4909 1752.9672
3765 877.8702 1753.7258
3766 585.5873 1753.7401
3767 877.9153 1753.8160
3768 878.3696 1754.7246
3769 585.9163 1754.7271
3771 878.9452 1755.8758
3773 879.9733 1757.9320
3774 586.9855 1757.9347
3775 880.8846 1759.7546
3776 588.6005 1762.7797
3777 588.9290 1763.7652
3778 441.9525 1763.7809
3779 588.9966 1763.9680
3780 883.3883 1764.7620
3781 589.2629 1764.7669
3782 442.1990 1764.7669
3783 589.3326 1764.9760
3785 589.9692 1766.8858
3786 591.0044 1769.9914
3787 591.0050 1769.9932
3788 591.0054 1769.9944
3789 591.2405 1770.6997
3790 591.3371 1770.9895
3792 592.3016 1773.8830
3793 887.9558 1773.8970
3794 444.7273 1774.8801
3798 593.9794 1778.9164
3799 445.7368 1778.9181
3800 593.9802 1778.9188
3802 595.2523 1782.7351
3803 596.6618 1786.9636
3804 448.2224 1788.8605
3805 597.2942 1788.8608
3808 597.6284 1789.8634
3809 895.9986 1789.9826
3810 896.0000 1789.9854
3812 597.6700 1789.9882
3813 598.2362 1791.6868
3814 598.5652 1792.6738
3815 449.1758 1792.6741
3816 449.1802 1792.6917
3817 598.5959 1792.7659
3818 598.6150 1792.8232
3819 449.4633 1793.8241
3820 599.3012 1794.8818
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