MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3509 705.3688 1408.7230
3511 705.8591 1409.7036
3512 470.9096 1409.7070
3513 470.9120 1409.7142
3514 709.8885 1417.7624
3515 710.8359 1419.6572
3518 356.1979 1420.7625
3519 356.1987 1420.7657
3523 712.3885 1422.7624
3525 475.5857 1423.7353
3526 475.8206 1424.4400
3527 357.1917 1424.7377
3528 357.4218 1425.6581
3529 713.8425 1425.6704
3530 713.8526 1425.6906
3531 713.8909 1425.7672
3533 476.5606 1426.6600
3555 357.9194 1427.6485
3556 477.6847 1430.0323
3557 477.6922 1430.0548
3558 478.2292 1431.6658
3559 716.8497 1431.6848
3560 478.9015 1433.6827
3562 718.8938 1435.7730
3563 719.3409 1436.6672
3564 481.2550 1440.7432
3565 361.4164 1441.6365
3566 481.5530 1441.6372
3568 481.5900 1441.7482
3569 481.9145 1442.7217
3570 362.9202 1447.6517
3571 483.5588 1447.6546
3577 484.5874 1450.7404
3578 726.8596 1451.7046
3579 727.3776 1452.7406
3580 485.2557 1452.7453
3581 485.2567 1452.7483
3582 364.1964 1452.7565
3583 485.2595 1452.7567
3584 485.2600 1452.7582
3589 367.1750 1464.6709
3590 489.2312 1464.6718
3591 489.2312 1464.6718
3592 733.3436 1464.6726
3593 367.1757 1464.6737
3594 733.8444 1465.6742
3595 489.9279 1466.7619
3596 734.3899 1466.7652
3597 734.8368 1467.6590
3598 490.2270 1467.6592
3599 736.3483 1470.6820
3600 491.2362 1470.6868
3601 737.3350 1472.6554
3602 737.3364 1472.6582
3603 737.8526 1473.6906
3605 493.2438 1476.7096
3606 494.2188 1479.6346
3607 494.2279 1479.6619
3608 494.5544 1480.6414
3609 741.3770 1480.7394
3610 495.5889 1483.7449
3611 495.5902 1483.7488
3613 495.5912 1483.7518
3614 742.9161 1483.8176
3615 743.3807 1484.7468
3617 496.5554 1486.6444
3619 498.5642 1492.6708
3620 374.1751 1492.6713
3621 747.3432 1492.6718
3623 748.3235 1494.6324
3624 751.8147 1501.6148
3625 751.8902 1501.7658
3626 376.6618 1502.6181
3627 501.8804 1502.6194
3628 752.8181 1503.6216
3629 377.1583 1504.6041
3630 754.4093 1506.8040
3633 755.3246 1508.6346
3634 503.8860 1508.6362
3635 756.3088 1510.6030
3636 756.3101 1510.6056
3637 760.3972 1518.7798
3638 765.3139 1528.6132
3639 765.8359 1529.6572
3640 510.8935 1529.6587
3641 765.8373 1529.6600
3642 511.2358 1530.6856
3643 511.2366 1530.6880
3644 511.2734 1530.7984
3646 770.4338 1538.8530
3648 774.8401 1547.6656
3649 774.8425 1547.6704
3651 518.8802 1553.6188
3653 782.9299 1563.8452
3655 523.5424 1567.6054
3656 392.9091 1567.6073
3657 393.1580 1568.6029
3658 797.4500 1592.8854
3659 801.8824 1601.7502
3661 814.4246 1626.8346
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