MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3324 659.7867 1317.5588
3325 662.2928 1322.5710
3328 662.3343 1322.6540
3330 441.8921 1322.6545
3333 662.3355 1322.6564
3334 441.8976 1322.6710
3335 662.3552 1322.6958
3338 443.7427 1328.2063
3342 666.2954 1330.5762
3343 666.3579 1330.7012
3344 667.2675 1332.5204
3345 445.1820 1332.5242
3346 668.8235 1335.6324
3349 447.1818 1338.5236
3350 670.2745 1338.5344
3352 670.7946 1339.5746
3353 670.8408 1339.6670
3354 447.8467 1340.5183
3356 673.2849 1344.5552
3357 449.1930 1344.5572
3358 449.2146 1344.6220
3359 449.2156 1344.6250
3360 449.5266 1345.5580
3361 673.8263 1345.6380
3362 337.4405 1345.7329
3368 337.4417 1345.7377
3369 676.3231 1350.6316
3372 451.2241 1350.6505
3373 676.3528 1350.6910
3374 451.5592 1351.6558
3375 677.2872 1352.5598
3376 452.5079 1354.5019
3377 678.2591 1354.5036
3378 678.7925 1355.5704
3379 678.7925 1355.5704
3383 454.5139 1360.5199
3384 681.2678 1360.5210
3385 341.1577 1360.6017
3386 681.3083 1360.6020
3387 454.5414 1360.6024
3388 341.1945 1360.7489
3389 681.7685 1361.5224
3390 341.3880 1361.5229
3391 454.8488 1361.5246
3392 341.4395 1361.7289
3393 455.2461 1362.7165
3395 682.8788 1363.7430
3410 341.9443 1363.7481
3413 341.9445 1363.7489
3414 341.9446 1363.7493
3419 342.1939 1364.7465
3420 342.1940 1364.7469
3421 683.3811 1364.7476
3427 684.3189 1366.6232
3428 456.5484 1366.6234
3429 456.9223 1367.7451
3430 457.1834 1368.5284
3431 685.2715 1368.5284
3432 457.8933 1370.6581
3433 686.3776 1370.7406
3435 688.8625 1375.7104
3436 688.8629 1375.7112
3437 459.5783 1375.7131
3438 460.1822 1377.5248
3441 345.4393 1377.7281
3443 460.5635 1378.6687
3446 460.9068 1379.6986
3449 460.9203 1379.7391
3450 460.9206 1379.7400
3452 460.9219 1379.7439
3455 462.2303 1383.6691
3456 693.8757 1385.7368
3457 463.5771 1387.7095
3459 463.5792 1387.7158
3462 695.3827 1388.7508
3463 464.2412 1389.7018
3464 464.2425 1389.7057
3465 696.8766 1391.7386
3467 464.9226 1391.7460
3469 465.2516 1392.7330
3472 465.2536 1392.7390
3473 465.2548 1392.7426
3475 465.5797 1393.7173
3476 697.8660 1393.7174
3477 465.5800 1393.7182
3479 465.5861 1393.7365
3483 466.2518 1395.7336
3484 698.8743 1395.7340
3486 698.8746 1395.7346
3487 466.2522 1395.7348
3490 466.2523 1395.7351
3493 466.2533 1395.7381
3494 466.2534 1395.7384
3495 698.8765 1395.7384
3501 468.9046 1403.6920
3502 468.9048 1403.6926
3503 469.9011 1406.6815
3504 470.2527 1407.7363
3506 704.8845 1407.7544
3508 470.5785 1408.7137
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