MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2827 315.1457 942.4153
2828 472.2159 942.4172
2829 472.2286 942.4426
2830 943.4513 942.4440
2831 472.7329 943.4512
2833 315.8363 944.4871
2838 474.2719 946.5292
2839 317.6606 949.9600
2840 476.2376 950.4606
2842 317.8346 950.4820
2845 318.4969 952.4689
2846 318.4971 952.4695
2848 477.2550 952.4954
2849 319.1527 954.4363
2850 955.4988 954.4915
2858 319.4905 955.4497
2860 478.7430 955.4714
2862 481.7329 961.4512
2863 483.2379 964.4612
2864 483.2386 964.4626
2865 484.2365 966.4584
2866 323.1605 966.4597
2867 323.4879 967.4419
2868 484.7297 967.4448
2870 485.2608 968.5070
2871 485.7536 969.4926
2873 486.2594 970.5042
2874 324.5091 970.5055
2875 324.8131 971.4175
2881 327.8044 980.3914
2882 491.2111 980.4076
2884 491.7213 981.4280
2885 492.2584 982.5022
2886 492.2590 982.5034
2887 328.5089 982.5049
2888 983.5336 982.5263
2889 492.2705 982.5264
2890 983.5339 982.5266
2891 983.5362 982.5289
2893 328.8141 983.4205
2894 328.8142 983.4208
2895 492.7383 983.4620
2896 493.2276 984.4406
2898 985.5167 984.5094
2899 493.2620 984.5094
2900 985.5176 984.5103
2901 493.2627 984.5108
2904 493.9460 985.8774
2912 495.5849 989.1552
2913 495.7255 989.4364
2921 331.8269 992.4589
2922 497.2368 992.4590
2923 497.6095 993.2044
2924 332.1581 993.4525
2925 332.2074 993.6004
2929 333.8320 998.4742
2930 500.2461 998.4776
2931 501.2372 1000.4598
2932 501.2507 1000.4868
2937 502.2664 1002.5182
2940 502.7601 1003.5056
2941 502.7609 1003.5072
2942 335.8093 1004.4061
2943 504.2807 1006.5468
2944 337.0004 1007.9794
2945 337.4978 1009.4716
2946 1010.4871 1009.4798
2950 338.1548 1011.4426
2952 338.1612 1011.4618
2953 506.7382 1011.4618
2956 507.2274 1012.4402
2959 339.1776 1014.5110
2961 508.7563 1015.4980
2962 1016.5057 1015.4984
2963 508.7565 1015.4984
2964 509.0053 1015.9960
2965 509.2399 1016.4652
2966 509.7372 1017.4598
2967 1020.5397 1019.5324
2968 510.7737 1019.5328
2970 341.4892 1021.4458
2971 512.7574 1023.5002
2972 342.8240 1025.4502
2974 342.8245 1025.4517
2976 342.8263 1025.4571
2978 344.1686 1029.4840
2979 515.7493 1029.4840
2980 344.1692 1029.4858
2982 344.4840 1030.4302
2983 516.3036 1030.5926
2984 516.7411 1031.4676
2986 345.4680 1033.3822
2987 518.7558 1035.4970
2988 518.7560 1035.4974
2989 518.7579 1035.5012
2990 518.7581 1035.5016
2991 346.1747 1035.5023
2993 346.8302 1037.4688
2995 520.2156 1038.4166
2996 520.7261 1039.4376
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