MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2656 369.6600 737.3054
2657 370.1920 738.3694
2658 743.3519 742.3446
2659 743.4451 742.4378
2660 372.6887 743.3628
2661 372.6893 743.3640
2663 374.1360 746.2574
2664 374.7046 747.3946
2665 748.4025 747.3952
2666 374.7054 747.3962
2667 375.1848 748.3550
2668 377.1580 752.3014
2669 755.3717 754.3644
2670 378.2067 754.3988
2671 757.3647 756.3574
2676 383.6359 765.2572
2677 772.3679 771.3606
2681 387.7112 773.4078
2682 782.4070 781.3997
2683 391.7073 781.4000
2684 392.2195 782.4244
2685 392.2198 782.4250
2686 393.1161 784.2176
2690 403.7313 805.4480
2694 406.7552 811.4958
2699 410.1794 818.3442
2701 411.7304 821.4462
2704 412.7374 823.4602
2705 412.7556 823.4966
2706 413.6956 825.3766
2713 418.7064 835.3982
2714 836.4633 835.4560
2715 836.4656 835.4583
2716 836.4695 835.4622
2717 419.7188 837.4230
2718 421.7053 841.3960
2720 842.5132 841.5059
2725 859.1018 858.0945
2726 430.7353 859.4560
2728 432.7587 863.5028
2729 433.7379 865.4612
2730 435.2689 868.5232
2732 435.7751 869.5356
2733 435.7769 869.5392
2734 871.9120 870.9047
2736 437.6796 873.3446
2738 877.0842 876.0769
2742 884.4615 883.4542
2745 886.4069 885.3996
2746 444.7199 887.4252
2753 446.7682 891.5218
2756 449.7260 897.4374
2757 449.7531 897.4916
2758 449.7734 897.5322
2762 301.6807 902.0203
2765 302.4985 904.4737
2766 453.2447 904.4748
2767 454.2320 906.4494
2769 304.8324 911.4754
2770 456.7452 911.4758
2771 304.8354 911.4844
2772 304.8355 911.4847
2773 456.7498 911.4850
2776 457.2256 912.4366
2777 457.2256 912.4366
2778 457.7398 913.4650
2779 457.7401 913.4656
2781 306.4739 916.3999
2782 459.2285 916.4424
2783 459.7578 917.5010
2784 918.5091 917.5018
2785 459.7582 917.5018
2786 459.7583 917.5020
2787 462.1947 922.3748
2788 462.1949 922.3752
2790 309.1494 924.4264
2791 463.2219 924.4292
2793 309.8007 926.3803
2794 464.1975 926.3804
2795 464.7331 927.4516
2796 310.1582 927.4528
2798 464.7349 927.4552
2802 310.5114 928.5124
2805 310.5119 928.5139
2810 465.9868 929.9590
2811 467.2415 932.4684
2812 467.2424 932.4702
2813 467.2440 932.4734
2814 312.1509 933.4309
2815 467.7427 933.4708
2816 312.8038 935.3896
2817 469.2445 936.4744
2818 313.1740 936.5002
2819 313.1742 936.5008
2820 313.8129 938.4169
2821 470.2163 938.4180
2822 471.2642 940.5138
2824 314.8530 941.5372
2825 314.8532 941.5378
2826 471.7762 941.5378
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