MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2479 304.1483 606.2820
2480 305.6175 609.2204
2481 305.6885 609.3624
2482 305.6889 609.3632
2483 308.1168 614.2190
2484 308.1169 614.2192
2485 311.0823 620.1500
2486 311.1478 620.2810
2489 312.6599 623.3052
2490 312.7144 623.4142
2491 313.0906 624.1666
2492 313.6855 625.3564
2493 626.3640 625.3567
2494 626.3641 625.3568
2495 315.1066 628.1986
2498 315.6835 629.3524
2504 319.6861 637.3576
2505 638.3651 637.3578
2506 319.6864 637.3582
2507 638.3657 637.3584
2508 321.2878 640.5610
2509 321.6651 641.3156
2510 321.6656 641.3166
2526 651.3510 650.3437
2527 653.3463 652.3390
2528 328.6631 655.3116
2529 329.6706 657.3266
2530 329.6707 657.3268
2532 660.3340 659.3267
2541 664.2534 663.2461
2542 332.6749 663.3352
2544 664.4144 663.4071
2550 668.8223 667.8150
2551 335.6712 669.3278
2554 336.1784 670.3422
2555 336.1789 670.3432
2557 672.3481 671.3408
2558 336.6778 671.3410
2559 336.6784 671.3422
2560 336.6787 671.3428
2561 336.6788 671.3430
2563 336.6892 671.3638
2565 337.1761 672.3376
2566 337.1830 672.3514
2569 675.3459 674.3386
2571 340.6931 679.3716
2572 340.6935 679.3724
2573 683.8774 682.8701
2574 342.6500 683.2854
2575 342.6568 683.2990
2577 342.6859 683.3572
2578 342.7073 683.4000
2579 343.6865 685.3584
2580 343.6867 685.3588
2581 343.7017 685.3888
2582 343.7026 685.3906
2583 344.1660 686.3174
2584 687.3366 686.3293
2586 345.1597 688.3048
2587 345.1598 688.3050
2591 346.6786 691.3426
2592 693.3483 692.3410
2596 348.6795 695.3444
2597 696.4065 695.3992
2598 348.9421 695.8696
2599 349.6445 697.2744
2600 349.6512 697.2878
2602 700.3651 699.3578
2603 350.6866 699.3586
2607 703.3025 702.2952
2610 352.6603 703.3060
2611 704.3643 703.3570
2613 353.6813 705.3480
2614 707.3873 706.3800
2615 707.4246 706.4173
2616 355.1379 708.2612
2617 709.3192 708.3119
2618 355.6521 709.2896
2619 355.6628 709.3110
2624 357.1528 712.2910
2625 357.1534 712.2922
2626 715.3554 714.3481
2627 358.1814 714.3482
2628 358.1815 714.3484
2629 358.1816 714.3486
2630 715.3560 714.3487
2632 359.6367 717.2588
2633 718.3276 717.3203
2635 720.3334 719.3261
2638 720.4072 719.3999
2639 360.7078 719.4010
2640 360.7078 719.4010
2641 720.4085 719.4012
2642 361.6916 721.3686
2643 725.3969 724.3896
2644 363.2025 724.3904
2646 364.1913 726.3680
2648 364.1922 726.3698
2649 364.1922 726.3698
2653 365.6472 729.2798
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