MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2358 479.2654 478.2581
2359 479.2669 478.2596
2360 479.2672 478.2599
2361 481.2632 480.2559
2362 484.2078 483.2005
2363 487.1511 486.1438
2364 493.0183 492.0110
2365 493.0204 492.0131
2366 493.0204 492.0131
2367 493.0207 492.0134
2368 493.0209 492.0136
2369 493.0213 492.0140
2370 493.0214 492.0141
2371 493.1171 492.1098
2372 495.2602 494.2529
2373 495.2603 494.2530
2374 495.2606 494.2533
2375 495.2610 494.2537
2376 496.2534 495.2461
2377 497.1723 496.1650
2378 497.5524 496.5451
2379 501.2377 500.2304
2380 502.2272 501.2199
2382 511.3041 510.2968
2383 513.2794 512.2721
2384 513.2799 512.2726
2385 514.2857 513.2784
2386 515.3330 514.3257
2387 519.2156 518.2083
2388 519.2473 518.2400
2389 519.3174 518.3101
2390 520.2576 519.2503
2391 524.2853 523.2780
2392 524.2853 523.2780
2393 524.2860 523.2787
2394 527.1824 526.1751
2395 528.2502 527.2429
2396 528.7712 527.7639
2397 529.2744 528.2671
2399 529.2756 528.2683
2400 529.2760 528.2687
2401 529.2761 528.2688
2402 529.2762 528.2689
2403 529.2763 528.2690
2407 529.3489 528.3416
2408 530.3309 529.3236
2409 531.1165 530.1092
2411 531.2915 530.2842
2413 533.1055 532.0982
2415 536.3109 535.3036
2416 536.3109 535.3036
2417 537.2338 536.2265
2418 537.2354 536.2281
2419 537.2366 536.2293
2420 537.7319 536.7246
2421 538.2310 537.2237
2422 538.2328 537.2255
2423 539.3311 538.3238
2426 542.2585 541.2512
2429 545.2712 544.2639
2432 550.2332 549.2259
2433 550.2682 549.2609
2434 550.2687 549.2614
2435 550.2693 549.2620
2436 551.2492 550.2419
2437 552.2803 551.2730
2438 552.2810 551.2737
2439 555.2648 554.2575
2440 559.2168 558.2095
2441 559.2666 558.2593
2442 561.2773 560.2700
2444 562.2610 561.2537
2445 563.3303 562.3230
2446 566.2632 565.2559
2447 566.2635 565.2562
2448 567.2446 566.2373
2449 567.2447 566.2374
2450 568.2416 567.2343
2451 568.2424 567.2351
2452 569.1529 568.1456
2453 569.3430 568.3357
2454 569.3434 568.3361
2455 572.2512 571.2439
2459 577.2792 576.2719
2461 578.2636 577.2563
2462 581.2601 580.2528
2463 581.2607 580.2534
2464 582.3085 581.3012
2465 584.2215 583.2142
2466 584.2297 583.2224
2467 588.9945 587.9872
2470 594.2329 593.2256
2471 594.3140 593.3067
2472 300.1893 598.3640
2473 301.1213 600.2280
2474 302.0932 602.1718
2475 302.6086 603.2026
2476 605.2729 604.2656
2477 303.5982 605.1818
2478 303.5985 605.1824
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