MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2701–2800 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2256 451.1940 450.1867
2257 451.1954 450.1881
2258 451.1958 450.1885
2259 451.1967 450.1894
2260 451.1972 450.1899
2261 451.1973 450.1900
2262 451.1979 450.1906
2263 451.2332 450.2259
2264 451.2333 450.2260
2265 451.2334 450.2261
2266 451.2341 450.2268
2267 451.2342 450.2269
2268 451.2347 450.2274
2269 451.2352 450.2279
2270 453.2107 452.2034
2271 453.2110 452.2037
2272 453.2118 452.2045
2273 453.2119 452.2046
2274 453.2120 452.2047
2275 453.2121 452.2048
2276 453.2125 452.2052
2277 453.2125 452.2052
2278 453.2126 452.2053
2279 453.2127 452.2054
2280 453.2128 452.2055
2281 453.2473 452.2400
2282 453.2481 452.2408
2283 453.2484 452.2411
2284 453.2589 452.2516
2285 453.2595 452.2522
2286 453.3450 452.3377
2287 455.1614 454.1541
2288 455.1913 454.1840
2289 455.2064 454.1991
2290 455.2266 454.2193
2291 455.2273 454.2200
2292 455.2283 454.2210
2293 455.2286 454.2213
2294 455.2286 454.2213
2295 457.1405 456.1332
2296 457.1420 456.1347
2297 457.2076 456.2003
2298 457.2253 456.2180
2299 457.2254 456.2181
2300 457.2260 456.2187
2301 457.2266 456.2193
2302 457.2783 456.2710
2303 458.2731 457.2658
2304 459.1201 458.1128
2305 459.2417 458.2344
2306 460.3148 459.3075
2307 461.2567 460.2494
2308 461.2568 460.2495
2309 461.2575 460.2502
2310 461.2752 460.2679
2311 463.1401 462.1328
2312 463.2336 462.2263
2313 463.2338 462.2265
2314 464.7476 463.7403
2315 465.2122 464.2049
2316 465.2132 464.2059
2317 465.2470 464.2397
2318 465.2496 464.2423
2319 465.2503 464.2430
2320 466.0446 465.0373
2321 467.0927 466.0854
2322 467.1615 466.1542
2323 467.2269 466.2196
2324 467.2269 466.2196
2325 467.2272 466.2199
2326 467.2278 466.2205
2327 468.2111 467.2038
2328 469.2408 468.2335
2329 469.2412 468.2339
2330 469.2419 468.2346
2331 469.2778 468.2705
2332 471.0943 470.0870
2333 471.1343 470.1270
2334 471.1557 470.1484
2335 472.2914 471.2841
2336 472.3257 471.3184
2337 472.3263 471.3190
2338 472.3276 471.3203
2339 472.7283 471.7210
2340 473.1099 472.1026
2341 473.1356 472.1283
2342 473.1505 472.1432
2343 473.2045 472.1972
2344 474.2684 473.2611
2345 474.2694 473.2621
2346 474.2695 473.2622
2347 474.2700 473.2627
2349 475.2355 474.2282
2350 475.2366 474.2293
2352 475.2907 474.2834
2353 475.3285 474.3212
2354 477.2520 476.2447
2355 479.2289 478.2216
2356 479.2637 478.2564
2357 479.2650 478.2577
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