MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1956 411.1648 410.1575
1957 411.1655 410.1582
1958 411.1659 410.1586
1959 411.2003 410.1930
1960 411.2004 410.1931
1961 411.2005 410.1932
1962 411.2007 410.1934
1963 411.2008 410.1935
1964 411.2008 410.1935
1965 411.2013 410.1940
1966 411.2015 410.1942
1967 411.2016 410.1943
1968 411.2019 410.1946
1969 411.2024 410.1951
1970 412.2299 411.2226
1971 413.1146 412.1073
1972 413.1152 412.1079
1973 413.1517 412.1444
1974 413.1802 412.1729
1975 413.1808 412.1735
1976 413.1811 412.1738
1977 413.1814 412.1741
1978 413.1817 412.1744
1979 413.2679 412.2606
1980 413.2687 412.2614
1981 413.2727 412.2654
1982 414.2476 413.2403
1983 415.0935 414.0862
1984 415.0946 414.0873
1985 415.1306 414.1233
1986 415.1318 414.1245
1987 415.1659 414.1586
1988 415.1964 414.1891
1989 416.2623 415.2550
1990 416.2629 415.2556
1991 416.2629 415.2556
1992 416.2639 415.2566
1993 416.2647 415.2574
1994 416.3757 415.3684
1995 416.3759 415.3686
1996 416.3764 415.3691
1997 417.2467 416.2394
1998 417.2474 416.2401
1999 417.2485 416.2412
2000 417.2487 416.2414
2001 419.1704 418.1631
2002 419.1963 418.1890
2003 419.2054 418.1981
2004 419.2061 418.1988
2005 419.2065 418.1992
2006 419.2067 418.1994
2007 419.2070 418.1997
2008 419.2072 418.1999
2009 419.2074 418.2001
2010 419.2075 418.2002
2011 419.2086 418.2013
2012 419.2440 418.2367
2013 419.2441 418.2368
2014 421.0588 420.0515
2015 421.1478 420.1405
2016 421.1846 420.1773
2017 421.1848 420.1775
2018 421.1851 420.1778
2019 421.1852 420.1779
2020 421.1854 420.1781
2021 421.1854 420.1781
2022 421.1862 420.1789
2023 421.1862 420.1789
2024 421.1863 420.1790
2025 421.1869 420.1796
2026 421.1871 420.1798
2027 421.2029 420.1956
2028 421.2034 420.1961
2029 421.2041 420.1968
2030 421.2045 420.1972
2031 421.2047 420.1974
2032 421.2047 420.1974
2033 421.2047 420.1974
2034 421.2049 420.1976
2035 421.2052 420.1979
2036 421.2209 420.2136
2037 421.2215 420.2142
2038 421.2216 420.2143
2039 421.2227 420.2154
2040 421.2227 420.2154
2041 421.2228 420.2155
2042 421.2228 420.2155
2043 421.2230 420.2157
2044 421.2231 420.2158
2045 421.2231 420.2158
2046 421.2234 420.2161
2047 423.0740 422.0667
2048 423.1356 422.1283
2049 423.1636 422.1563
2050 423.1638 422.1565
2051 423.1654 422.1581
2052 423.1654 422.1581
2053 423.1656 422.1583
2054 423.1997 422.1924
2055 423.1999 422.1926
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