MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2301–2400 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1856 405.1911 404.1838
1857 405.1913 404.1840
1858 405.1913 404.1840
1859 405.1914 404.1841
1860 405.1915 404.1842
1861 405.1915 404.1842
1862 405.1916 404.1843
1863 405.2101 404.2028
1864 405.2156 404.2083
1865 405.2270 404.2197
1866 405.2270 404.2197
1867 405.2280 404.2207
1868 405.2285 404.2212
1869 407.1317 406.1244
1870 407.1320 406.1247
1871 407.1688 406.1615
1872 407.1691 406.1618
1873 407.1691 406.1618
1874 407.1693 406.1620
1875 407.1694 406.1621
1876 407.1694 406.1621
1877 407.1696 406.1623
1878 407.1696 406.1623
1879 407.1703 406.1630
1880 407.1706 406.1633
1881 407.1706 406.1633
1882 407.2054 406.1981
1883 407.2057 406.1984
1884 407.2061 406.1988
1885 407.2062 406.1989
1886 407.2063 406.1990
1887 407.2066 406.1993
1888 407.2066 406.1993
1889 407.2067 406.1994
1890 407.2068 406.1995
1891 407.2070 406.1997
1892 407.2071 406.1998
1893 407.2074 406.2001
1894 407.2078 406.2005
1895 407.2079 406.2006
1896 407.2086 406.2013
1897 407.2236 406.2163
1898 407.2241 406.2168
1899 407.2247 406.2174
1900 407.2249 406.2176
1901 407.2250 406.2177
1902 407.2252 406.2179
1903 407.2253 406.2180
1904 407.2256 406.2183
1905 407.2256 406.2183
1906 407.2261 406.2188
1907 407.2430 406.2357
1908 409.0847 408.0774
1909 409.1479 408.1406
1910 409.1492 408.1419
1911 409.1496 408.1423
1912 409.1500 408.1427
1913 409.1647 408.1574
1914 409.1841 408.1768
1915 409.1848 408.1775
1916 409.1848 408.1775
1917 409.1848 408.1775
1918 409.1850 408.1777
1919 409.1853 408.1780
1920 409.1854 408.1781
1921 409.1854 408.1781
1922 409.1859 408.1786
1923 409.1861 408.1788
1924 409.1861 408.1788
1925 409.1861 408.1788
1926 409.1862 408.1789
1927 409.1863 408.1790
1928 409.1865 408.1792
1929 409.1865 408.1792
1930 409.2213 408.2140
1931 409.2215 408.2142
1932 409.2218 408.2145
1933 409.2223 408.2150
1934 409.2225 408.2152
1935 409.2228 408.2155
1936 409.2231 408.2158
1937 409.2231 408.2158
1938 409.2390 408.2317
1939 409.2398 408.2325
1940 409.2408 408.2335
1941 409.2409 408.2336
1942 409.2409 408.2336
1943 409.2411 408.2338
1944 410.2437 409.2364
1945 410.2438 409.2365
1946 410.2444 409.2371
1947 410.2449 409.2376
1948 411.0720 410.0647
1949 411.0997 410.0924
1950 411.1334 410.1261
1951 411.1625 410.1552
1952 411.1635 410.1562
1953 411.1643 410.1570
1954 411.1647 410.1574
1955 411.1648 410.1575
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