MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1756 393.3000 392.2927
1757 393.3000 392.2927
1758 393.3001 392.2928
1759 393.3002 392.2929
1760 393.3003 392.2930
1761 393.3003 392.2930
1762 393.3003 392.2930
1763 393.3004 392.2931
1764 393.3004 392.2931
1765 393.3006 392.2933
1766 393.3007 392.2934
1767 395.0768 394.0695
1768 395.0776 394.0703
1769 395.1687 394.1614
1770 395.1689 394.1616
1771 395.1691 394.1618
1772 395.1692 394.1619
1773 395.1693 394.1620
1774 395.1695 394.1622
1775 395.1695 394.1622
1776 395.1696 394.1623
1777 395.1697 394.1624
1778 395.1698 394.1625
1779 395.1700 394.1627
1780 395.1704 394.1631
1781 395.1704 394.1631
1782 395.1705 394.1632
1783 395.1705 394.1632
1784 395.1707 394.1634
1785 395.1707 394.1634
1786 395.1707 394.1634
1787 395.2050 394.1977
1788 395.2054 394.1981
1789 395.2054 394.1981
1790 395.2057 394.1984
1791 395.2063 394.1990
1792 395.2063 394.1990
1793 395.2066 394.1993
1794 395.2066 394.1993
1795 395.2067 394.1994
1796 395.2067 394.1994
1797 395.2072 394.1999
1798 395.2256 394.2183
1799 395.2640 394.2567
1800 397.0833 396.0760
1801 397.1196 396.1123
1802 397.1495 396.1422
1803 397.1496 396.1423
1804 397.1497 396.1424
1805 397.1502 396.1429
1806 397.1840 396.1767
1807 397.1841 396.1768
1808 397.1846 396.1773
1809 397.1848 396.1775
1810 397.1851 396.1778
1811 397.1853 396.1780
1812 397.1857 396.1784
1813 397.1857 396.1784
1814 397.1859 396.1786
1815 397.1859 396.1786
1816 397.1859 396.1786
1817 397.1860 396.1787
1818 397.1860 396.1787
1819 397.1861 396.1788
1820 397.1862 396.1789
1821 397.1864 396.1791
1822 397.1866 396.1793
1823 397.2206 396.2133
1824 397.2211 396.2138
1825 397.2216 396.2143
1826 397.2829 396.2756
1827 399.0995 398.0922
1828 399.0999 398.0926
1829 399.1522 398.1449
1830 399.2007 398.1934
1831 399.2008 398.1935
1832 399.2010 398.1937
1833 399.2010 398.1937
1834 399.2018 398.1945
1835 399.2018 398.1945
1836 399.2019 398.1946
1837 399.2215 398.2142
1838 399.2354 398.2281
1839 401.0777 400.0704
1840 401.1146 400.1073
1841 401.1152 400.1079
1842 401.1154 400.1081
1843 401.2363 400.2290
1844 401.2882 400.2809
1845 401.2884 400.2811
1846 402.2477 401.2404
1847 402.2482 401.2409
1848 403.1759 402.1686
1849 403.2329 402.2256
1850 404.2058 403.1985
1851 405.1903 404.1830
1852 405.1905 404.1832
1853 405.1907 404.1834
1854 405.1908 404.1835
1855 405.1910 404.1837
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