MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2101–2200 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1656 387.0980 386.0907
1657 387.0989 386.0916
1658 387.1359 386.1286
1659 387.1999 386.1926
1660 387.2002 386.1929
1661 387.2003 386.1930
1662 387.2003 386.1930
1663 387.2017 386.1944
1664 387.2018 386.1945
1665 387.2018 386.1945
1666 387.2043 386.1970
1667 387.2378 386.2305
1668 387.2387 386.2314
1669 388.2326 387.2253
1670 389.0527 388.0454
1671 389.1508 388.1435
1672 389.1597 388.1524
1673 389.1964 388.1891
1674 389.1966 388.1893
1675 389.1972 388.1899
1676 389.1972 388.1899
1677 391.1740 390.1667
1678 391.1744 390.1671
1679 391.1745 390.1672
1680 391.1746 390.1673
1681 391.1751 390.1678
1682 391.1754 390.1681
1683 391.1757 390.1684
1684 391.1758 390.1685
1685 391.1759 390.1686
1686 391.2111 390.2038
1687 391.2119 390.2046
1688 391.2123 390.2050
1689 391.2124 390.2051
1690 391.2124 390.2051
1691 391.2126 390.2053
1692 391.2128 390.2055
1693 391.2308 390.2235
1694 391.2863 390.2790
1695 391.2865 390.2792
1696 393.0893 392.0820
1697 393.1522 392.1449
1698 393.1527 392.1454
1699 393.1529 392.1456
1700 393.1531 392.1458
1701 393.1532 392.1459
1702 393.1537 392.1464
1703 393.1538 392.1465
1704 393.1538 392.1465
1705 393.1538 392.1465
1706 393.1544 392.1471
1707 393.1546 392.1473
1708 393.1550 392.1477
1709 393.1895 392.1822
1710 393.1897 392.1824
1711 393.1899 392.1826
1712 393.1900 392.1827
1713 393.1901 392.1828
1714 393.1902 392.1829
1715 393.1903 392.1830
1716 393.1905 392.1832
1717 393.1906 392.1833
1718 393.1907 392.1834
1719 393.1908 392.1835
1720 393.1911 392.1838
1721 393.1911 392.1838
1722 393.1913 392.1840
1723 393.1913 392.1840
1724 393.1916 392.1843
1725 393.1916 392.1843
1726 393.2104 392.2031
1727 393.2119 392.2046
1728 393.2284 392.2211
1729 393.2468 392.2395
1730 393.2469 392.2396
1731 393.2495 392.2422
1732 393.2986 392.2913
1733 393.2987 392.2914
1734 393.2987 392.2914
1735 393.2987 392.2914
1736 393.2987 392.2914
1737 393.2988 392.2915
1738 393.2990 392.2917
1739 393.2990 392.2917
1740 393.2990 392.2917
1741 393.2991 392.2918
1742 393.2991 392.2918
1743 393.2991 392.2918
1744 393.2992 392.2919
1745 393.2992 392.2919
1746 393.2994 392.2921
1747 393.2995 392.2922
1748 393.2996 392.2923
1749 393.2996 392.2923
1750 393.2996 392.2923
1751 393.2997 392.2924
1752 393.2998 392.2925
1753 393.2998 392.2925
1754 393.3000 392.2927
1755 393.3000 392.2927
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