MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2001–2100 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1556 379.2122 378.2049
1557 379.2123 378.2050
1558 379.2126 378.2053
1559 381.1529 380.1456
1560 381.1530 380.1457
1561 381.1535 380.1462
1562 381.1538 380.1465
1563 381.1539 380.1466
1564 381.1541 380.1468
1565 381.1541 380.1468
1566 381.1542 380.1469
1567 381.1542 380.1469
1568 381.1543 380.1470
1569 381.1546 380.1473
1570 381.1548 380.1475
1571 381.1548 380.1475
1572 381.1890 380.1817
1573 381.1893 380.1820
1574 381.1894 380.1821
1575 381.1897 380.1824
1576 381.1897 380.1824
1577 381.1898 380.1825
1578 381.1899 380.1826
1579 381.1900 380.1827
1580 381.1901 380.1828
1581 381.1905 380.1832
1582 381.1906 380.1833
1583 381.1907 380.1834
1584 381.1908 380.1835
1585 381.1908 380.1835
1586 381.1908 380.1835
1587 381.1909 380.1836
1588 381.1909 380.1836
1589 381.1910 380.1837
1590 381.1912 380.1839
1591 381.1912 380.1839
1592 381.1915 380.1842
1593 381.1918 380.1845
1594 381.2105 380.2032
1595 381.2106 380.2033
1596 381.2107 380.2034
1597 383.1031 382.0958
1598 383.1031 382.0958
1599 383.1316 382.1243
1600 383.1337 382.1264
1601 383.1344 382.1271
1602 383.1685 382.1612
1603 383.1688 382.1615
1604 383.1691 382.1618
1605 383.1691 382.1618
1606 383.1692 382.1619
1607 383.1693 382.1620
1608 383.1693 382.1620
1609 383.1695 382.1622
1610 383.1698 382.1625
1611 383.1701 382.1628
1612 383.1701 382.1628
1613 383.1703 382.1630
1614 383.1703 382.1630
1615 383.1711 382.1638
1616 383.2053 382.1980
1617 383.2055 382.1982
1618 383.2057 382.1984
1619 383.2066 382.1993
1620 383.2068 382.1995
1621 383.2069 382.1996
1622 383.2070 382.1997
1623 383.2070 382.1997
1624 383.2073 382.2000
1625 383.2075 382.2002
1626 383.2262 382.2189
1627 383.2264 382.2191
1628 383.2270 382.2197
1629 385.0831 384.0758
1630 385.0832 384.0759
1631 385.1190 384.1117
1632 385.1201 384.1128
1633 385.1364 384.1291
1634 385.1481 384.1408
1635 385.1567 384.1494
1636 385.1839 384.1766
1637 385.1842 384.1769
1638 385.1848 384.1775
1639 385.1852 384.1779
1640 385.1857 384.1784
1641 385.1858 384.1785
1642 385.1859 384.1786
1643 385.1861 384.1788
1644 385.1862 384.1789
1645 385.1862 384.1789
1646 385.1863 384.1790
1647 385.1863 384.1790
1648 385.2024 384.1951
1649 385.2036 384.1963
1650 385.2217 384.2144
1651 385.2567 384.2494
1652 385.2572 384.2499
1653 385.2578 384.2505
1654 385.2580 384.2507
1655 386.2408 385.2335
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