MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1456 371.3180 370.3107
1457 371.3181 370.3108
1458 371.3181 370.3108
1459 371.3182 370.3109
1460 371.3182 370.3109
1461 371.3183 370.3110
1462 371.3183 370.3110
1463 371.3183 370.3110
1464 371.3184 370.3111
1465 371.3188 370.3115
1466 372.0525 371.0452
1467 372.1941 371.1868
1468 372.1942 371.1869
1469 373.0828 372.0755
1470 373.1201 372.1128
1471 373.2213 372.2140
1472 373.2223 372.2150
1473 374.2927 373.2854
1474 374.2928 373.2855
1475 374.2932 373.2859
1476 375.1062 374.0989
1477 375.1065 374.0992
1478 375.1436 374.1363
1479 375.1807 374.1734
1480 375.1807 374.1734
1481 375.1812 374.1739
1482 375.1812 374.1739
1483 375.1997 374.1924
1484 375.2000 374.1927
1485 375.2005 374.1932
1486 375.2007 374.1934
1487 375.2011 374.1938
1488 375.2170 374.2097
1489 375.2176 374.2103
1490 375.2364 374.2291
1491 375.2368 374.2295
1492 376.2335 375.2262
1493 376.2354 375.2281
1494 377.1212 376.1139
1495 377.1213 376.1140
1496 377.1581 376.1508
1497 377.1584 376.1511
1498 377.1585 376.1512
1499 377.1588 376.1515
1500 377.1588 376.1515
1501 377.1588 376.1515
1502 377.1589 376.1516
1503 377.1594 376.1521
1504 377.1595 376.1522
1505 377.1598 376.1525
1506 377.1598 376.1525
1507 377.1601 376.1528
1508 377.1603 376.1530
1509 377.1950 376.1877
1510 377.1956 376.1883
1511 377.1960 376.1887
1512 377.1960 376.1887
1513 377.1962 376.1889
1514 377.1963 376.1890
1515 377.1963 376.1890
1516 377.1964 376.1891
1517 377.1964 376.1891
1518 377.1966 376.1893
1519 377.1967 376.1894
1520 378.0260 377.0187
1521 379.0999 378.0926
1522 379.1380 378.1307
1523 379.1381 378.1308
1524 379.1381 378.1308
1525 379.1386 378.1313
1526 379.1389 378.1316
1527 379.1390 378.1317
1528 379.1394 378.1321
1529 379.1737 378.1664
1530 379.1738 378.1665
1531 379.1738 378.1665
1532 379.1741 378.1668
1533 379.1742 378.1669
1534 379.1744 378.1671
1535 379.1744 378.1671
1536 379.1744 378.1671
1537 379.1745 378.1672
1538 379.1749 378.1676
1539 379.1752 378.1679
1540 379.1752 378.1679
1541 379.1752 378.1679
1542 379.1753 378.1680
1543 379.1753 378.1680
1544 379.1754 378.1681
1545 379.1754 378.1681
1546 379.1756 378.1683
1547 379.1758 378.1685
1548 379.1758 378.1685
1549 379.1944 378.1871
1550 379.2102 378.2029
1551 379.2113 378.2040
1552 379.2115 378.2042
1553 379.2116 378.2043
1554 379.2116 378.2043
1555 379.2120 378.2047
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